Cores in foot-and-mouth disease virus
| Autor: | M. S. Dubra, Claudio D. Denoya, J. L. La Torre, C. Vasquez, E.A. Scodeller |
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| Rok vydání: | 1982 |
| Předmět: |
Genes
Viral ON-glycerol Biology Acetates law.invention chemistry.chemical_compound Aphthovirus law Virology Freezing Centrifugation Density Gradient Incubation Virion Hydrogen-Ion Concentration biology.organism_classification Microscopy Electron Dextran sulfate Biochemistry chemistry Biophysics Nucleic Acid Conformation RNA Viral Ultracentrifuge Electron microscope Foot-and-mouth disease virus Ammonium acetate |
| Zdroj: | Virology. 116(1) |
| ISSN: | 0042-6822 |
| Popis: | Foot-and-mouth disease virions were dissociated with ammonium acetate and observed with the electron microscope. The major products of viral disassembly were 12 S viral subunits or skullcaps and cores. Cores appeared as spherical structures and were relatively unstable upon spreading, freezing and thawing, or treatment at low pH. Upon addition of dextran sulfate to the incubation mixture, it was possible to analyze cores by ultracentrifugation on glycerol gradients. Cores sedimented at 45 S but large amounts of filamentous structures, possibly unfolded cores, were also found at slower rates. The results of this work indicated that cores are predominantly formed by genomic RNA folded in a compact spherical configuration. |
| Databáze: | OpenAIRE |
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