Gene expression during redifferentiation of human articular chondrocytes
| Autor: | Camilla Karlsson, Andreas Brunner, Anders Lindahl, Josefine van der Lee, Rupert Hagg, Tommi Tallheden, Roberto Tommasini |
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| Rok vydání: | 2004 |
| Předmět: |
Adult
Cartilage Articular Redifferentiation Dermatopontin Biomedical Engineering Gene Expression Microarray Chondrocyte Chondrocytes Downregulation and upregulation Rheumatology Matrix gla protein medicine Humans Orthopedics and Sports Medicine Cells Cultured Oligonucleotide Array Sequence Analysis biology Cartilage Tenascin C Cell Differentiation Autologous cell transplantation Chondrogenesis Immunohistochemistry Molecular biology Fibronectin medicine.anatomical_structure biology.protein Articular chondrocytes Collagen |
| Zdroj: | Osteoarthritis and Cartilage. 12(7):525-535 |
| ISSN: | 1063-4584 |
| DOI: | 10.1016/j.joca.2004.03.004 |
| Popis: | Objective The aim of the present study was to investigate gene expression during the in vitro redifferentiation process of human articular chondrocytes isolated from clinical samples from patient undergoing an autologous chondrocyte transplantation therapy (ACT). Method Monolayer (ML) expanded human articular chondrocytes from four donors were cultured in a 3D pellet model and the redifferentiation was investigated by biochemistry, histology, immunohistochemistry and microarray analysis. Results The culture expanded chondrocytes redifferentiated in the pellet model as seen by an increase in collagen type II immunoreactivity between day 7 and 14. The gene expression from ML to pellet at day 7 included an increase in cartilage matrix proteins like collagen type XI, tenascin C, dermatopontin, COMP and fibronectin. The late phase consisted of a strong downregulation of extracellular signal-regulated protein kinase (ERK-1) and an upregulation of p38 kinase and SOX-9, suggesting that the late phase mimicked parts of the signaling processes involved in the early chondrogenesis in limb bud cells. Other genes, which indicated a transition from proliferation to tissue formation, were the downregulated cell cycle genes GSPT1 and the upregulated growth-arrest-specific protein (gas). The maturation of the pellets included no signs of hypertrophy or apoptosis as seen by downregulation of collagen type X, Matrix Gla protein and increased expression of caspase 3. Conclusion Our data show that human articular chondrocytes taken from surplus cells of patient undergoing ACT treatment and expanded in ML, redifferentiate and form cartilage like matrix in vitro and that this dynamic process involves genes known to be expressed in early chondrogenesis. |
| Databáze: | OpenAIRE |
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