Manganese mixture inhalation is a reliable Parkinson disease model in rats
| Autor: | Verónica Anaya-Martínez, Leonardo Reynoso-Erazo, José Luis Ordoñez-Librado, Ana Luisa Gutierrez-Valdez, Jesús Espinosa-Villanueva, Maria Rosa Avila-Costa, Enrique Montiel-Flores, Javier Sánchez-Betancourt |
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| Rok vydání: | 2012 |
| Předmět: |
Male
Pathology medicine.medical_specialty Tyrosine 3-Monooxygenase Video Recording chemistry.chemical_element Striatum Manganese Motor Activity Toxicology Antiparkinson Agents Levodopa Mice Dopamine Internal medicine Administration Inhalation medicine Animals Rats Wistar Neurologic Examination Analysis of Variance Inhalation Pars compacta Chemistry General Neuroscience Manganese Poisoning Dopaminergic Neurotoxicity Brain Parkinson Disease Feeding Behavior medicine.disease Rats Disease Models Animal Endocrinology Globus pallidus nervous system Manganese Compounds Phosphopyruvate Hydratase Locomotion Psychomotor Performance medicine.drug |
| Zdroj: | Neurotoxicology. 33(5) |
| ISSN: | 1872-9711 |
| Popis: | Manganese (Mn) is an essential trace metal. Regardless of its essentiality, it has been reported that the overexposure causes neurotoxicity manifested as extrapyramidal symptoms similar to those observed in Parkinson disease (PD). Recently, our group reported that mice that inhaled for 5 months the mixture of manganese chloride (MnCl(2)) and manganese acetate Mn(OAc)(3) developed movement abnormalities, significant loss of substantia nigra compacta (SNc) dopaminergic neurons, dopamine depletion and improved behavior with l-DOPA treatment. However, this model has only been characterized in mice. In order to have a well-supported and generalizable model in rodents, we used male Wistar rats that inhaled a mixture of 0.04 M MnCl(2) and 0.02 M Mn(OAc)(3), 1h three times a week for 6 months. Before Mn exposure, animals were trained to perform motor tests (Beam-walking and Single-pellet reaching tasks) and were evaluated each week after the exposure. The mixture of MnCl(2)/Mn(OAc)(3) caused alterations in the motor tests, 75.95% loss of SNc dopaminergic neurons, and no cell alterations in Globus Pallidus or striatum. With these results we conclude that the inhalation of the mixture of Mn compounds is a useful model in rodents for the study of PD. |
| Databáze: | OpenAIRE |
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