A monoclonal antibody against LAV gag precursor: use for viral protein analysis and antigenic expression in infected cells

Autor:	Chassagne J, Verrelle P, Dionet C, Clavel F, Barre-Sinoussi F, Jc, Chermann, Montagnier L, Jc, Gluckman, David Klatzmann
Rok vydání:	1986
Předmět:	Acquired Immunodeficiency Syndrome Mice Inbred BALB C Hybridomas T-Lymphocytes Immunology Retroviridae Proteins Antibodies Monoclonal Gene Products gag Lymphocyte Activation Deltaretrovirus Molecular Weight Mice Animals Immunology and Allergy Female Protein Precursors Antigens Viral Tumor
Zdroj:	Europe PubMed Central
ISSN:	1550-6606 0022-1767
DOI:	10.4049/jimmunol.136.4.1442
Popis:	A murine monoclonal antibody (MAb), named C.V.K., was produced after immunization with highly purified and sonicated lymphadenopathy-associated virus (LAV). No monoclonal antibody was observed with intact virus used as immunogen. C.V.K. MAb recognizes an epitope present on the precursor gag protein of 55 kilodaltons. Western blot analysis and pulse-chase experiments support the interpretation that after p55 cleavage into p25, p18, and p13, only p18 expresses this epitope. C.V.K. MAb selectively stained only LAV-infected lymphocytes. This intracytoplasmic staining appears 3 days after the infection and is correlated with reverse transcriptase activity. Neither membrane immunofluorescence of infected lymphocytes nor neutralizing activity was observed with C.V.K. MAb. These facts suggest that p55 and p18 are not expressed at the cell membrane or on the viral envelope. C.V.K. MAb should prove useful not only in the purification of core proteins but also for detecting infected cells producing the virus in suspension or on histologic sections.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_dedup___::aa357010bbe00a46e79185cf59250634 https://doi.org/10.4049/jimmunol.136.4.1442 Zobrazit plný text záznamu