Wide-field and two-photon imaging of brain activity with voltage- and calcium-sensitive dyes
| Autor: | Lawrence B. Cohen, Arthur Konnerth, Olga Garaschuk, Bradley J. Baker, Marco Canepari, Ryota Homma, Dejan Zecevic, Maja Djurisic, Lei Jin, Chun X. Bleau |
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| Přispěvatelé: | Department of Cellular and Molecular Physiology, Yale School of Medicine, Institute of Neuroscience, Technical University of Munich ( TUM ), RedShirtImaging, LLC, DC05259/DC/NIDCD NIH HHS/United States NS42739/NS/NINDS NIH HHS/United States, Fahmeed Hyder, Yale University School of Medicine, Technische Universität Munchen - Université Technique de Munich [Munich, Allemagne] (TUM) |
| Jazyk: | angličtina |
| Rok vydání: | 2009 |
| Předmět: |
MESH: Photons
Spike train Population Biology Noise (electronics) MESH: Brain 03 medical and health sciences 0302 clinical medicine MESH : Photons Two-photon excitation microscopy MESH : Coloring Agents [SDV.MHEP.PHY]Life Sciences [q-bio]/Human health and pathology/Tissues and Organs [q-bio.TO] medicine MESH: Animals education MESH : Calcium 030304 developmental biology 0303 health sciences education.field_of_study MESH: Humans Olfactory receptor MESH : Evoked Potentials [ SDV.MHEP.PHY ] Life Sciences [q-bio]/Human health and pathology/Tissues and Organs [q-bio.TO] MESH : Humans Shot noise Animals Brain/*physiology Calcium/*chemistry Coloring Agents/*administration & dosage Evoked Potentials Humans *Photons Olfactory bulb MESH: Evoked Potentials Light intensity medicine.anatomical_structure MESH : Brain MESH: Calcium Biophysics MESH : Animals MESH: Coloring Agents 030217 neurology & neurosurgery |
| Zdroj: | Methods in Molecular Biology (Clifton then Totova) Dynamic Brain Imaging: Multi-Modal Methods and In Vivo Applications Fahmeed Hyder. Dynamic Brain Imaging: Multi-Modal Methods and In Vivo Applications, 489, Humana Press Springer, pp.43-79, 2009, Methods in Molecular Biology, 〈10.1007/978-1-59745-543-5_3〉 Methods in Molecular Biology ISBN: 9781934115749 Methods in Molecular Biology Fahmeed Hyder. Dynamic Brain Imaging: Multi-Modal Methods and In Vivo Applications, 489, Humana Press Springer, pp.43-79, 2009, Methods in Molecular Biology, ⟨10.1007/978-1-59745-543-5_3⟩ |
| ISSN: | 1064-3745 1940-6029 |
| DOI: | 10.1007/978-1-59745-543-5_3〉 |
| Popis: | International audience; This chapter presents three examples of imaging brain activity with voltage- or calcium-sensitive dyes. Because experimental measurements are limited by low sensitivity, the chapter then discusses the methodological aspects that are critical for optimal signal-to-noise ratio. Two of the examples use wide-field (1-photon) imaging and the third uses two-photon scanning microscopy. These methods have relatively high temporal resolution ranging from 10 to 10,000 Hz. The three examples are the following: (1) Internally injected voltage-sensitive dye can be used to monitor membrane potential in the dendrites of invertebrate and vertebrate neurons in in vitro preparations. These experiments are directed at understanding how individual neurons convert the complex input synaptic activity into the output spike train. (2) Recently developed methods for staining many individual cells in the mammalian brain with calcium-sensitive dyes together with two-photon microscopy made it possible to follow the spike activity of many neurons simultaneously while in vivo preparations are responding to stimulation. (3) Calcium-sensitive dyes that are internalized into olfactory receptor neurons in the nose will, after several days, be transported to the nerve terminals of these cells in the olfactory bulb glomeruli. There, the population signals can be used as a measure of the input from the nose to the bulb. Three kinds of noise in measuring light intensity are discussed: (1) Shot noise from the random emission of photons from the preparation. (2) Extraneous (technical) noise from external sources. (3) Noise that occurs in the absence of light, the dark noise. In addition, we briefly discuss the light sources, the optics, and the detectors and cameras. The commonly used organic voltage and ion sensitive dyes stain all of the cell types in the preparation indiscriminately. A major effort is underway to find methods for staining individual cell types in the brain selectively. Most of these efforts center around fluorescent protein activity sensors because transgenic methods can be used to express them in individual cell types. |
| Databáze: | OpenAIRE |
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