Inhibition of type 5 adenovirus infectivity by periodate oxidation
| Autor: | V. Thomas, J. D. Rodwell, G. Ogier, Yvonne Michal, G. Quash |
|---|---|
| Rok vydání: | 1994 |
| Předmět: |
DNA Replication
Base pair viruses Restriction Mapping Biology medicine.disease_cause Antiviral Agents Virus Restriction fragment chemistry.chemical_compound Virology medicine Humans Infectivity chemistry.chemical_classification Viral Structural Proteins Adenoviruses Human Periodic Acid Periodate General Medicine Molecular biology Adenoviridae Cross-Linking Reagents chemistry Biochemistry DNA Viral biology.protein Electrophoresis Polyacrylamide Gel Glycoprotein Oxidation-Reduction DNA HeLa Cells Thymidine |
| Zdroj: | Archives of virology. 135(1-2) |
| ISSN: | 0304-8608 |
| Popis: | Periodate oxidation of purified type 5 Adenovirus (Ad5) led to a mean loss of infectivity of 6.84 logs. There were no significant differences in adsorption and penetration between oxidized and mock-oxidized virus. However, after infection with oxidized virus, no synthesis of viral structural proteins could be detected and a 78.5% inhibition of viral DNA synthesis was observed. Labelling experiments performed by treating oxidized and mock-oxidized virus with tritiated sodium borohydride revealed that the fiber glycoprotein was one of the proteins labelled in oxidized virus whereas no labelled proteins were detected in non oxidized virus. In addition, it was found that one mol of formaldehyde generated during oxidation of sugar residues was bound per 500 base pairs in oxidized virus. One consequence of this in situ generation of formaldehyde is the formation of DNA-protein crosslinks. The DNA so crosslinked showed different patterns of restriction fragments with endonucleases such as Hpa I, Hind III and Kpn I but not with Xho I. |
| Databáze: | OpenAIRE |
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