Low prevalence of hepatitis E virus in the liver of Corsican pigs slaughtered after 12 months despite high antibody seroprevalence

Autor: Marie Pellerin, Bastien Trabucco, Lisandru Capai, Morgane Laval, Oscar Maestrini, Ferran Jori, Alessandra Falchi, Virginie Doceul, François Charrier, François Casabianca, Nicole Pavio
Přispěvatelé: Virologie UMR1161 (VIRO), École nationale vétérinaire - Alfort (ENVA)-Laboratoire de santé animale, sites de Maisons-Alfort et de Normandie, Agence nationale de sécurité sanitaire de l'alimentation, de l'environnement et du travail (ANSES)-Agence nationale de sécurité sanitaire de l'alimentation, de l'environnement et du travail (ANSES)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), Systèmes d'Elevage Méditerranéens et Tropicaux - Laboratoire de Recherche sur le Développement de l'Elevage (SELMET-LRDE), Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), EA Bioscope Corse Méditerranée : Dynamique des infections virales en milieu insulaire, Université Pascal Paoli (UPP)-Institut National de la Santé et de la Recherche Médicale (INSERM), Charité - UniversitätsMedizin = Charité - University Hospital [Berlin], Animal, Santé, Territoires, Risques et Ecosystèmes (UMR ASTRE), Centre de Coopération Internationale en Recherche Agronomique pour le Développement (Cirad)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), Département Systèmes Biologiques (Cirad-BIOS), Centre de Coopération Internationale en Recherche Agronomique pour le Développement (Cirad), Laboratoire Interdisciplinaire Sciences, Innovations, Sociétés (LISIS), Centre National de la Recherche Scientifique (CNRS)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)-Université Gustave Eiffel, Doceul, Virginie
Rok vydání: 2022
Předmět:
Zdroj: Transboundary and Emerging Diseases
Transboundary and emerging diseases
Transboundary and emerging diseases, 2022, 69 (5), pp.e2706-e2718. ⟨10.1111/tbed.14621⟩
ISSN: 1865-1682
1865-1674
Popis: International audience; Hepatitis E virus (HEV) infection can be acute and benign or evolve to chronic hepatitis with rapid progression toward cirrhosis or liver failure in humans. Hence, Hepatitis E (HE) disease is a major public health concern. In countries where pig populations are highly contaminated with HEV, human cases of HE are mainly foodborne, occurring frequently after consumption of raw or undercooked pork products or liver. Among factors associated to the presence of HEV in pork livers from intensive rearing systems, early slaughter (≤6 months) seems to be major. In Corsica, local pigs are raised in extensive farming systems and slaughtered after 12 months. To evaluate if slaughter of pigs over 12 months reduces the risk of HEV presence in livers, 1 197 liver samples were randomly collected in 2 Corsican slaughterhouses. Presence of HEV RNA was detected in liver and HEV seroprevalence was determined in paired serum. The sampling included 1,083 livers from animals between 12 and 48 months and 114 livers from animals < 12 months. The samples were predominantly from semi-extensive and extensive farms (n = 1 ,154). Estimated HEV seroprevalence was high, > 88% and HEV RNA prevalence in adult pig livers (>12-month-old) was low 0.18 %. However, in livers from younger animals (< 12 months), including piglets below 6-month-old, 5.3% (6/114) of the samples were positive for HEV RNA. Sequences recovered from positive livers belonged to HEV genotype 3c and 3f. The presence of infectious HEV was confirmed in 2 livers by the detection of HEV replication in HepaRG cell cultures. Thus, this study, demonstrates the low prevalence of HEV in livers of pigs over 12 months, even in farms with high HEV circulation. This observation may open new perspectives on the preferential use of livers from animals older than 12 months in raw pork liver products.
Databáze: OpenAIRE
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