Catalase Protects Biofilm of Staphylococcus aureus against Daptomycin Activity
Autor: | Mads Lichtenberg, Thomas Bjarnsholt, Karen Leth Nielsen, Peter Østrup Jensen, Cristina El Haj |
---|---|
Jazyk: | angličtina |
Rok vydání: | 2021 |
Předmět: |
0301 basic medicine
Microbiology (medical) Staphylococcus aureus daptomycin 030106 microbiology RM1-950 medicine.disease_cause Biochemistry Microbiology Article biofilm 03 medical and health sciences Daptomycin medicine polycyclic compounds Pharmacology (medical) General Pharmacology Toxicology and Pharmaceutics chemistry.chemical_classification Reactive oxygen species biology Chemistry Biofilm catalase Wild type biochemical phenomena metabolism and nutrition biology.organism_classification Catalase bacterial infections and mycoses carbohydrates (lipids) 030104 developmental biology Infectious Diseases Mechanism of action biology.protein lipids (amino acids peptides and proteins) Therapeutics. Pharmacology medicine.symptom Bacteria medicine.drug |
Zdroj: | Antibiotics, Vol 10, Iss 511, p 511 (2021) Antibiotics Volume 10 Issue 5 El Haj, C, Lichtenberg, M, Nielsen, K L, Bjarnsholt, T & Jensen, P Ø 2021, ' Catalase protects biofilm of Staphylococcus aureus against daptomycin activity ', Antibiotics, vol. 10, no. 5, 511 . https://doi.org/10.3390/antibiotics10050511 |
ISSN: | 2079-6382 |
Popis: | Daptomycin is recommended for the treatment of Staphylococcus aureus infections due to its bactericidal activity. However, its mechanism of action is poorly understood. The involvement of reactive oxygen species (ROS) in the bactericidal activity of daptomycin has been proved against planktonic S. aureus, but not against the biofilm of S. aureus. Therefore, we evaluated if ROS contributes to the effect of daptomycin against biofilm of S. aureus. Biofilms of wild type, catalase deficient and daptomycin-resistant S. aureus strains were grown in microtiter-plates. After three days, the biofilms were exposed to daptomycin with or without thiourea in the presence of a ROS indicator. After overnight incubation, the amount of ROS and the percentage of surviving bacteria were determined. The bacterial survival was higher and the amount of ROS was lower in the wild type than in the catalase deficient biofilm, demonstrating a protective effect of catalase against daptomycin. The induction of cytotoxic ROS formation by daptomycin was verified by the addition of thiourea, which reduced the amount of ROS and protected the wild type biofilm against high concentrations of daptomycin. Accordingly, only the highest concentration of daptomycin reduced the bacterial survival and increased the ROS formation in the resistant biofilm. In conclusion, daptomycin induced the production of cytotoxic levels of endogenous ROS in S. aureus biofilm and the presence of catalase protected the biofilm against the lethality of the induced ROS. |
Databáze: | OpenAIRE |
Externí odkaz: |