Integrating morphological characters, molecular markers, and distribution patterns to assess the identity of Blepharis species from Jordan
Autor: | Mohammad Brake, Muheeb Awawdeh, Mazhar Salim Al Zoubi, Robert I. Colautti, Khalid M. Al-Batayneh, Jamil N. Lahham, Riyadh Muhaidat, Athena D. McKown, Wesam Al Khateeb, Amjad D. Al-Nasser, Ahmad El-Oqlah |
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Jazyk: | angličtina |
Rok vydání: | 2018 |
Předmět: |
0106 biological sciences
Linear discriminant analysis Population Dimorphic stamens Spike Plant Science 010603 evolutionary biology 01 natural sciences Floristics Genus lcsh:Botany Botany Blepharis ciliaris Inflorescence education Bract education.field_of_study biology Blepharis Inter-Simple Sequence Repeat Blepharis attenuata biology.organism_classification lcsh:QK1-989 Genetic marker Key (lock) Original Article 010606 plant biology & botany |
Zdroj: | Botanical Studies, Vol 59, Iss 1, Pp 1-15 (2018) Botanical Studies |
ISSN: | 1999-3110 |
DOI: | 10.1186/s40529-018-0234-x |
Popis: | Background: Blepharis constitutes an important part of the vegetation of the Jordanian arid and semi-arid regions, yet whether one or more species of this genus occurs in the Jordanian area is uncertain. We addressed this question by assessing morphological characters and testing Inter-Simple Sequence Repeat (ISSR) markers from three populations of Blepharis: two northern (lower slopes of Kufranjah valley and the Dead Sea region) and one southern (Wadi al Yutm). Results: Shoots from randomly chosen Blepharis plants were harvested from each of the three populations for morphological and molecular analyses. In the northern populations, spikes were lax and bract width was significantly shorter than length of the longest lateral spine compared to the southern population. A multivariate linear discriminant analysis distinguished the northern populations from the southern one by internode length, bract width, longest lateral spine length, and bract width to spine length ratio. The ISSR analysis revealed that 44 markers across eight primers were polymorphic with major allele frequency of 83.6% and an average of 5.5 polymorphic markers per primer. The genetic resemblance among individuals ranged from 0.27 to 0.96. The three Blepharis populations were accordingly clustered into two distinct groups, similar to the analysis of morphological differences and corresponding with the “northern” and “southern” population designations. Conclusions: Our results strongly indicate the occurrence of two discrete Blepharis species in Jordan and reject the hypothesis that the genus is represented by only one species. We propose that the Blepharis species in Jordan are B. attenutata Napper (represented by the northern populations) and B. ciliaris (L.) B. L. Burtt (represented by the southern population). These findings are important for informing and revising floristic work within the region and an updated key has been included in our findings. |
Databáze: | OpenAIRE |
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