Validation of Multiplex Serology detecting human herpesviruses 1-5
| Autor: | Naomi E. Allen, Allison E. Aiello, J Brozy, Nicole Brenner, Michael Pawlita, Angelika Michel, K Prager, Benedikt Weißbrich, Tim Waterboer, Meier Hcs., R Almond, Alexander J. Mentzer, Julia Butt, Judith Breuer |
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| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
Male Physiology viruses medicine.disease_cause Pathology and Laboratory Medicine Antibodies Viral Biochemistry Serology Database and Informatics Methods 0302 clinical medicine Immune Physiology Medicine and Health Sciences Multiplex Enzyme-Linked Immunoassays Prospective cohort study Child Antigens Viral Herpesviridae Aged 80 and over education.field_of_study Multidisciplinary Immune System Proteins virus diseases Kaposi's Sarcoma-Associated Herpesvirus Herpesviridae Infections Middle Aged Recombinant Proteins 3. Good health Medical Microbiology 030220 oncology & carcinogenesis Viral Pathogens Child Preschool Viruses Medicine Female Pathogens Sequence Analysis Encephalitis Research Article Adult Herpesviruses Adolescent Bioinformatics Science Population Immunology Congenital cytomegalovirus infection Sequence Databases Research and Analysis Methods Microbiology Virus Antibodies 03 medical and health sciences Young Adult medicine Epstein-Barr virus Humans Serologic Tests Antigens education Immunoassays Microbial Pathogens Aged business.industry Varicella zoster virus Organisms Biology and Life Sciences Proteins Infant medicine.disease Virology High-Throughput Screening Assays 030104 developmental biology Biological Databases Immunologic Techniques business DNA viruses |
| Zdroj: | PLoS ONE, Vol 13, Iss 12, p e0209379 (2018) PLoS ONE |
| DOI: | 10.17615/jnxr-3613 |
| Popis: | Human herpesviruses (HHV) cause a variety of clinically relevant conditions upon primary infection of typically young and immunocompetent hosts. Both primary infection and reactivation after latency can lead to more severe disease, such as encephalitis, congenital defects and cancer. Infections with HHV are also associated with cardiovascular and neuro-degenerative disease. However, most of the associations are based on retrospective case-control analyses and well-powered prospective cohort studies are needed for assessing temporality and causality. To enable comprehensive investigations of HHV-related disease etiology in large prospective population-based cohort studies, we developed HHV Multiplex Serology. This methodology represents a low-cost, high-throughput technology that allows simultaneous measurement of specific antibodies against five HHV species: Herpes simplex viruses 1 and 2, Varicella zoster virus, Epstein-Barr virus, and Cytomegalovirus. The newly developed HHV species-specific (‘Monoplex’) assays were validated against established gold-standard reference assays. The specificity and sensitivity of the HHV species-specific Monoplex Serology assays ranged from 92.3% to 100.0% (median 97.4%) and 91.8% to 98.7% (median 96.6%), respectively. Concordance with reference assays was very high with kappa values ranging from 0.86 to 0.96 (median kappa 0.93). Multiplexing the Monoplex Serology assays resulted in no loss of performance and allows simultaneous detection of antibodies against the 5 HHV species in a high-throughput manner. |
| Databáze: | OpenAIRE |
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