Reliability of low-avidity IgG and of IgA in the diagnosis of primary infection by rubella virus with adaptation of a commercial test
| Autor: | Gonzalo Piédrola, F. De Ory, María del Carmen Maroto, José Gutiérrez, M. Rodríguez |
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| Rok vydání: | 1999 |
| Předmět: |
Adult
Microbiology (medical) Indirect elisa Clinical Biochemistry Group ii Antibody Affinity Acute infection Enzyme-Linked Immunosorbent Assay chemical and pharmacologic phenomena Antibodies Viral medicine.disease_cause medicine Humans Immunology and Allergy Avidity Rubella Low avidity biology Biochemistry (medical) Public Health Environmental and Occupational Health Rubella virus Original Articles Hematology Igg avidity Immunoglobulin A Medical Laboratory Technology Immunoglobulin G Immunology biology.protein Female Reagent Kits Diagnostic Antibody |
| Zdroj: | Journal of Clinical Laboratory Analysis. 13:1-4 |
| ISSN: | 1098-2825 0887-8013 |
| DOI: | 10.1002/(sici)1098-2825(1999)13:1<1::aid-jcla1>3.0.co;2-z |
| Popis: | 2National Health Centers for Microbiology, Virology, and Immunology, Madrid, Spain The detection of IgA and low-avidity IgG and antibodies in serum is a potentially useful marker of recent infection by a microorganism. We studied the reliability of IgG avidity and presence of IgA for the diagnosis of recent acute infection by rubella virus. Low-avidity IgG (Avy-EIA test) was determined with a modified commercial test using 8 molar urea (indirect ELISA, DiaSorin, Italy) and IgA was determined with a homemade indirect ELISA test. Twenty-five patients with recent primary infection by rubella virus (group I) and 50 healthy subjects (group II) were studied. In group I low-avidity IgG varied between 100 and 0% (67.3 ± 21.8%); IgA was present in 24 patients (96%). In group II low-avidity IgG varied from 50.4 to 0% (19.8 ± 16.9%). IgA was present in 2 subjects (4%). The sensitivity of the Avi-EIA and the IgA test was 92 and 96%, respectively; specificity was 100 and 96%, respectively. We conclude that both low-avidity IgG and IgA tests are helpful and reliable for the diagnosis of recent primary infection. J. Clin. Lab. Anal. 13:1‐4, 1999. © 1999 Wiley-Liss, Inc. |
| Databáze: | OpenAIRE |
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