Redox and metabolic strategies developed by anterior and posterior gills of the crab Neohelice granulata after short periods of hypo- or hyper-osmotic stress
| Autor: | F.A. Fernandes, Anapaula Sommer Vinagre, Francisca Mosele, G.D. Ferreira, Adriane Belló-Klein, Luiz Carlos Rios Kucharski, B.K. Dutra, Alex Sander da Rosa Araujo, R.S.M. Da Silva |
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| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
Gill Gills medicine.medical_specialty animal structures Environmental Engineering Osmotic shock Brachyura medicine.disease_cause Excretion 03 medical and health sciences chemistry.chemical_compound Osmotic Pressure Stress Physiological Internal medicine medicine Environmental Chemistry Animals Waste Management and Disposal Neohelice biology Chemistry Glutathione Hydrogen Peroxide biology.organism_classification Pollution Hsp70 030104 developmental biology Endocrinology Catalase biology.protein Sodium-Potassium-Exchanging ATPase Oxidation-Reduction Oxidative stress |
| Zdroj: | The Science of the total environment. 639 |
| ISSN: | 1879-1026 |
| Popis: | The aim of this study was to identify the response pattern of redox balance, Na+/K+ATPase activity and HSP70 expression in the posterior and anterior gills of the crab Neohelice granulata submitted to hypo- or hyper-osmotic stress for 1 h and 6 h. After 1 h of either type of osmotic stress, there was an increase in catalase activity, but a decrease in GSSG/GSH ratio (oxidized to reduced glutathione ratio) and Na+/K+ATPase activity in both gill sets. H2O2 levels decreased only in the posterior gills. H2O2 levels and Na+/K+ATPase activity remained reduced after 6 h of exposure to either type of osmotic stress in both gill sets. The GSSG/GSH ratio returned to initial levels after 6 h of hyper-osmotic stress, whereas it increased 10 times in both gill sets after hypo-osmotic stress. Furthermore, HSP70 protein expression increased in posterior gills after 6 h of hypo-osmotic stress. H2O2 levels in tank water decreased after hypo-osmotic challenge and increased after 6 h of hyper-osmotic stress, indicating increased H2O2 excretion. Therefore, N. granulata gills have redox, metabolic and molecular strategies to deal with rapid osmotic challenges, an important environmental parameter that influences juvenile and adult crab distribution and abundance within different populations. |
| Databáze: | OpenAIRE |
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