Bacterial Compound N,N-Dimethylhexadecylamine Modulates Expression of Iron Deficiency and Defense Response Genes in Medicago truncatula Independently of the Jasmonic Acid Pathway
| Autor: | Ernesto García-Pineda, Vicente Montejano-Ramírez, Eduardo Valencia-Cantero |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
0106 biological sciences
0301 basic medicine salicylic acid cross-talk in stress-response pathways Plant Science 01 natural sciences Article 03 medical and health sciences chemistry.chemical_compound lcsh:Botany Gene expression Pseudomonas syringae Gene Ecology Evolution Behavior and Systematics iron deprivation Botrytis cinerea Ecology biology Jasmonic acid fungi food and beverages biology.organism_classification Medicago truncatula lcsh:QK1-989 030104 developmental biology chemistry Biochemistry Alternative complement pathway bacterial organic volatile compound Salicylic acid 010606 plant biology & botany |
| Zdroj: | Plants Volume 9 Issue 5 Plants, Vol 9, Iss 624, p 624 (2020) |
| ISSN: | 2223-7747 |
| DOI: | 10.3390/plants9050624 |
| Popis: | Plants face a variety of biotic and abiotic stresses including attack by microbial phytopathogens and nutrient deficiencies. Some bacterial volatile organic compounds (VOCs) activate defense and iron-deficiency responses in plants. To establish a relationship between defense and iron deficiency through VOCs, we identified key genes in the defense and iron-deprivation responses of the legume model Medicago truncatula and evaluated the effect of the rhizobacterial VOC N,N-dimethylhexadecylamine (DMHDA) on the gene expression in these pathways by RT-qPCR. DMHDA increased M. truncatula growth 1.5-fold under both iron-sufficient and iron-deficient conditions compared with untreated plants, whereas salicylic acid and jasmonic acid decreased growth. Iron-deficiency induced iron uptake and defense gene expression. Moreover, the effect was greater in combination with DMHDA. Salicylic acid, Pseudomonas syringae, jasmonic acid, and Botrytis cinerea had inhibitory effects on growth and iron response gene expression but activated defense genes. Taken together, our results showed that the VOC DMHDA activates defense and iron-deprivation pathways while inducing a growth promoting effect unlike conventional phytohormones, highlighting that DMHDA does not mimic jasmonic acid but induces an alternative pathway. This is a novel aspect in the complex interactions between biotic and abiotic stresses. |
| Databáze: | OpenAIRE |
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