Improved growth performance due to hypertrophied intestinal absorptive epithelial cells by heat-killed Lactobacillus sakei HS-1 in broiler chickens1
| Autor: | Duddoa Khonyoung, Koh-en Yamauchi |
|---|---|
| Rok vydání: | 2019 |
| Předmět: |
Male
Hot Temperature Cell Ileum Weight Gain Andrology 03 medical and health sciences Basal (phylogenetics) Latilactobacillus sakei Stress Physiological Genetics medicine Animals Intestinal Mucosa Gizzard 030304 developmental biology 0303 health sciences biology Chemistry Villus Tip Body Weight 0402 animal and dairy science Broiler Epithelial Cells Hypertrophy 04 agricultural and veterinary sciences General Medicine biology.organism_classification Animal Feed 040201 dairy & animal science Small intestine Anti-Bacterial Agents Diet Lactobacillus sakei Intestines medicine.anatomical_structure Feeds Dietary Supplements Animal Science and Zoology Chickens Food Science |
| Zdroj: | J Anim Sci |
| ISSN: | 1525-3163 0021-8812 |
| Popis: | This study investigated the effect of heat-killed Lactobacillus sakei HS-1 (HK LS HS-1) on the growth performance and intestinal histology of broilers through 2 feeding trials. In trial 1, 48 male broilers were separated into 3 groups: 1) basal diet (1 to 21 d, including antibiotics; 21 to 49 d, not including antibiotics) supplemented with 0 bacterial cells of HK LS HS-1/g (control); 2) 10(6) bacterial cells were used; and 3) 10(8) bacterial cells were used. Trial 2 was carried out in a tropical area of Thailand, where 50 7-d-old male broilers were separated into 2 groups: 1) basal diet (control group); and 2) basal diet supplemented with 10(7) bacterial cells of HK LS HS-1/g. In trial 1, compared with the control, BW gain (linear; P = 0.019) and G:F (linear; P = 0.032) linearly increase with increasing HK LS HS-1 supplementation. In addition, 3 males died in the control group, while none died in the experimental group. In trial 2, growth performance was not significantly different between the groups. Observation of the gross anatomical visceral organs and intestinal histological parameters showed no difference among the groups. However, the weight of the ileum, total small intestine, gizzard, and ceca decreased, while the height of the ileal villus increased with increasing HK LS HS-1 supplementation (P < 0.05). On the duodenal villus apical surface, protuberated cells, cells without microvilli, recently exfoliated regions on villus tips, and deeper cells at the sites of these recently exfoliated cells were more frequently observed in the experimental groups compared to the control; therefore, the duodenal epithelial cells of the experimental groups were hypertrophied by rapid cell turnover. On the ileal villus apical surface, morphological changes (i.e., cell shedding) were not observed, but protuberated cells were observed; thus, the ileal epithelial cells of the experimental groups were hypertrophied by prolonging the detention period of cells on the villus tip without shedding into the intestinal lumen. The results indicate that HK LS HS-1 improves the growth performance of broilers due to the presences of hypertrophied intestinal absorptive epithelial cells on the villus apical surface, which induce enhanced durability against environmental stress. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|