Nrl Is Dispensable for Specification of Rod Photoreceptors in Adult Zebrafish Despite Its Deeply Conserved Requirement Earlier in Ontogeny
| Autor: | W. Ted Allison, Spencer D. Balay, Keon Collett, A. Phillip Oel, Emily M. Dong, Gavin J. Neil |
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| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
0301 basic medicine
genetic structures Transgene 02 engineering and technology Biology Article Photoreceptor cell 03 medical and health sciences medicine Rod cell lcsh:Science Zebrafish Transcription factor Molecular Biology Retina Multidisciplinary Biological Sciences 021001 nanoscience & nanotechnology biology.organism_classification Cone cell Cell biology 030104 developmental biology medicine.anatomical_structure lcsh:Q sense organs 0210 nano-technology Developmental biology Developmental Biology |
| Zdroj: | iScience, Vol 23, Iss 12, Pp 101805-(2020) iScience |
| ISSN: | 2589-0042 |
| Popis: | Summary The transcription factor NRL (neural retina leucine zipper) has been canonized as the master regulator of photoreceptor cell fate in the retina. NRL is necessary and sufficient to specify rod cell fate and to preclude cone cell fate in mice. By engineering zebrafish, we tested if NRL function has conserved roles beyond mammals or beyond nocturnal species, i.e., in a vertebrate possessing a greater and more typical diversity of cone sub-types. Transgenic expression of Nrl from zebrafish or mouse was sufficient to induce rod photoreceptor cells. Zebrafish nrl−/− mutants lacked rods (and had excess UV-sensitive cones) as young larvae; thus, the conservation of Nrl function between mice and zebrafish appears sound. Strikingly, however, rods were abundant in adult nrl−/− null mutant zebrafish. Rods developed in adults despite Nrl protein being undetectable. Therefore, a yet-to-be-revealed non-canonical pathway independent of Nrl is able to specify the fate of some rod photoreceptors. Graphical Abstract Highlights • Nrl is conserved and sufficient to specify rod photoreceptors in the zebrafish retina • Nrl is necessary for rod photoreceptors in early ontogeny of zebrafish larvae • Zebrafish Nrl is functionally conserved with mouse and human NRL • Remarkably, Nrl is dispensable for rod specification in adult zebrafish Biological Sciences; Molecular Biology; Developmental Biology |
| Databáze: | OpenAIRE |
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