Stability of clavulanic acid in PEG/citrate and liquid–liquid extraction in aqueous two-phase system
| Autor: | José A. Teixeira, C. S. Porto, André Mota, Tatiana Souza Porto, M. N. Carneiro-da-Cunha, K. P. S. Souza, Ana Lúcia Figueiredo Porto |
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| Přispěvatelé: | Universidade do Minho |
| Jazyk: | angličtina |
| Rok vydání: | 2014 |
| Předmět: |
Aqueous solution
Chromatography Science & Technology Chemistry General Chemical Engineering Extraction (chemistry) Citrate salts Aqueous two-phase system General Physics and Astronomy Clavulanic acid PEG ATPS Hydrolysis chemistry.chemical_compound Liquid–liquid extraction PEG ratio medicine Lactam Physical and Theoretical Chemistry Stability medicine.drug |
| Zdroj: | Repositório Científico de Acesso Aberto de Portugal Repositório Científico de Acesso Aberto de Portugal (RCAAP) instacron:RCAAP |
| Popis: | β-Lactamases are enzymes responsible for the hydrolysis of β-lactam antibiotics, being produced by several pathogenic bacteria. Clavulanic acid is a commercially and clinically important β-lactamase inhibitor, its extraction being possible by the application of aqueous two-phase system. In this study, clavulanic acid stability was investigated at different molar mass PEG (400, 1 000 and 20 000 g mol−1) and at different citrate concentrations (5 and 20%) PEG/citrate aqueous-two phase systems (ATPS), under different pH values (4.0–8.0). Clavulanic acid extraction was also evaluated. Low citrate concentration and PEG 20 000 (g mol−1) ATPS, at pH 6.0, were shown to be the systems that presented the highest clavulanic acid stability. Based on this, a factorial design 22 was used to evaluate CA extraction, being PEG (20, 25 and 30%) and citrate (5, 10 and 15%) concentrations the parameters evaluated. Clavulanic acid was extracted into the PEG-rich phase (133.66 mg L−1), the highest values of the partition coefficient and yield being K = 5.92, Y = 103.53%, respectively The ATPS was not only effective on clavulanic acid extraction, but also its degradation was minimal. These results clearly indicate that ATPS can be successfully applied as a first step for the purification of clavulanic acid. CNPq -Conselho Nacional de Desenvolvimento Científico e Tecnológico(150763/2013-1) |
| Databáze: | OpenAIRE |
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