Neuronatin mRNA in PC12 cells: downregulation by nerve growth factor
| Autor: | Wayne Tsang, Dexian Dou, Joseph R, Kevin Nelson, Klaus Edvardsen |
|---|---|
| Rok vydání: | 1996 |
| Předmět: |
Aging
RNA Splicing Basic fibroblast growth factor Nerve Tissue Proteins Biology PC12 Cells chemistry.chemical_compound Exon Downregulation and upregulation Epidermal growth factor Animals Nerve Growth Factors RNA Messenger Cycloheximide Molecular Biology Nucleic Acid Synthesis Inhibitors Protein Synthesis Inhibitors Messenger RNA General Neuroscience Membrane Proteins Molecular biology Rats Nerve growth factor chemistry Dactinomycin Fibroblast Growth Factor 2 Neuronatin Neurology (clinical) Developmental Biology Transforming growth factor |
| Zdroj: | Brain Research. 738:32-38 |
| ISSN: | 0006-8993 |
| DOI: | 10.1016/0006-8993(96)00768-8 |
| Popis: | Neuronatin was recently cloned from neonatal rat brain (Biochem, Biophys. Res. Commun., 201 (1994) 1227-1234). In subsequent studies, we noted neuronatin mRNA was brain-specific and that there were two alternatively spliced forms, alpha and beta (Brain Res., 690 (1995) 92-98). Furthermore, on sequencing the human neuronatin gene, it was determined that the alpha-form was encoded by three exons, and the beta-form was encoded by the first and third exons only (Genomics, 33 (1996) 292-297). The middle exon was spliced out in the beta-form. The human neuronatin gene is located in single copy of chromosome 20q 11.2-12 (Brain Res., 723 (1996) 8-22). These studies called for an understanding of the function of this gene. Therefore, we studied the expression of neuronatin in PC12 cells, an established model of neuronal growth and differentiation. Neuronatin mRNA expression was found to be abundant in undifferentiated PC12 cells. Treatment with nerve growth factor (NGF), resulting in neuronal differentiation, was associated with a downregulation of neuronatin mRNA expression. Removal of NGF was associated with a return of neuronatin mRNA levels towards baseline. These effects appear to be specific for NGF as they were not seen with transforming growth factor, epidermal growth factor, 12-O-tetradecanoylphorbol-13-acetate or dexamethasone. Although, basic fibroblast growth factor also reduced neuronatin mRNA levels, the effect was less pronounced than with NGF. The NGF-induced decreased in neuronatin mRNA occurred even in the presence of protein and RNA syntheses inhibitors. Of the two spliced forms, only the alpha-form was expressed in PC12 cells. In conclusion, we report the presence of neuronatin mRNA in PC12 cells, and that NGF downregulates its expressions. These findings provide a basis for investigating the role of neuronatin in neuronal growth and differentiation. |
| Databáze: | OpenAIRE |
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