Heterogeneity of nanomechanical properties of the human umbilical vein endothelial cell surface
| Autor: | Nastassia M. Shkliarava, Aleksey N. Kondrachyk, Marina S. Nedoseikina, Maria N. Starodubtseva, Eldar A. Nadyrov, Matsvei V. Matveyenkau, I. E. Starodubtsev, Alena U. Tsukanava |
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| Rok vydání: | 2021 |
| Předmět: |
0301 basic medicine
Endothelium Cell 030204 cardiovascular system & hematology Microscopy Atomic Force Mechanotransduction Cellular Biochemistry Umbilical vein 03 medical and health sciences 0302 clinical medicine Elastic Modulus Human Umbilical Vein Endothelial Cells medicine Humans Nanotechnology Cells Cultured Cytoskeleton Cell Nucleus Chemistry Atomic force microscopy Cell Membrane Significant difference Adhesiveness Cell Biology Endothelial stem cell Phenotype 030104 developmental biology medicine.anatomical_structure Biophysics Human umbilical vein endothelial cell Cell structure Stress Mechanical Single-Cell Analysis Cardiology and Cardiovascular Medicine |
| Zdroj: | Microvascular Research. 136:104168 |
| ISSN: | 0026-2862 |
| DOI: | 10.1016/j.mvr.2021.104168 |
| Popis: | Endothelial cells, due to heterogeneity in the cell structure, can potentially form an inhomogeneous on structural and mechanical properties of the inner layer of the capillaries. Using quantitative nanomechanical mapping mode of atomic force microscopy, the parameters of the structural, elastic, and adhesive properties of the cell surface for living and glutaraldehyde-fixed human umbilical vein endothelial cells were studied. A significant difference in the studied parameters for three cell surface zones (peripheral, perinuclear, and nuclear zones) was established. The perinuclear zone appeared to be the softest zone of the endothelial cell surface. The heterogeneity of the endothelial cell mechanical properties at the nanoscale level can be an important mechanism in regulating the endothelium functions in blood vessels. |
| Databáze: | OpenAIRE |
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