Label-Free Assessment of Collagenase Digestion on Bovine Pericardium Properties by Fluorescence Lifetime Imaging
| Autor: | Jeny Shklover, Leigh G. Griffiths, Laura Marcu, Anne K. Haudenschild, Benjamin E. Sherlock, Mojtaba Parvizi, Cai Li, Alba Alfonso García |
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| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
In situ Fluorescence-lifetime imaging microscopy Biomedical Engineering Bioengineering Regenerative Medicine 01 natural sciences Regenerative medicine Fluorescence lifetime imaging Medical and Health Sciences Article 010309 optics Extracellular matrix 03 medical and health sciences Engineering 0103 physical sciences Ultimate tensile strength medicine Animals Collagenases Collagenase degradation Chemistry Optical Imaging Non-destructive monitoring Biomaterial Fluorescence Extracellular Matrix 030104 developmental biology Biophysics Collagenase Cattle Pericardium medicine.drug Biotechnology |
| Zdroj: | Annals of biomedical engineering, vol 46, iss 11 Ann Biomed Eng |
| Popis: | The extracellular matrix architecture of bovine pericardium (BP) has distinct biochemical and biomechanical properties that make it a useful biomaterial in the field of regenerative medicine. Collagen represents the dominant structural protein of BP and is therefore intimately associated with the properties of this biomaterial. Enzymatic degradation of collagen molecules is critical for extracellular matrix turnover, remodeling and ultimately tissue regeneration. We present a quantitative, label-free and non-destructive method for monitoring changes in biochemical and biomechanical properties of BP during tissue degradation, based on multi-spectral fluorescence lifetime imaging (ms-FLIm). Strong correlations of fluorescence intensity ratio and average fluorescence lifetime were identified with collagen content, Young's Modulus and Ultimate tensile strength during collagenase degradation, indicating the potential of optically monitoring collagen degradation using ms-FLIm. The obtained results demonstrate the value of ms-FLIm to assess the quality of biomaterials in situ for applications in regenerative medicine. |
| Databáze: | OpenAIRE |
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