Antibody repertoire development in fetal and neonatal piglets. XXII. λ rearrangement precedes κ rearrangement during B-cell lymphogenesis in swine
Autor: | John E. Butler, Katerina Stepanova, Marek Sinkora, Kelly M. Lager, Nancy Wertz, Xiu-Zhu Sun, Gregory J. Tobin |
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Rok vydání: | 2012 |
Předmět: |
Transcription
Genetic Swine Molecular Sequence Data Immunology Gene Rearrangement B-Lymphocyte Heavy Chain Biology Fetus Antibody Repertoire Antibody Specificity medicine Animals Immunology and Allergy Amino Acid Sequence Yolk sac B cell B-Lymphocytes Lymphopoiesis Gene Expression Regulation Developmental Original Articles Molecular biology Peripheral blood Junctional diversity medicine.anatomical_structure Animals Newborn Organ Specificity Antibody Formation Female Bone marrow Sequence Alignment |
Zdroj: | Immunology. 137:149-159 |
ISSN: | 0019-2805 |
DOI: | 10.1111/j.1365-2567.2012.03615.x |
Popis: | VDJ and VJ rearrangements, expression of RAG-1, Tdt and VpreB, and the presence of signal joint circles (SJC) were used to identify sites of B-cell lymphogenesis. VDJ, VλJλ but not VκJκ rearrangements or SJC were recovered from yolk sac (YS) at 20 days of gestation (DG) along with strong expression of VpreB and RAG-1 but weak Tdt expression. VλJλ rearrangements but not VκJκ rearrangements were recovered from fetal liver at 30-50 DG. SJC were pronounced in bone marrow at 95 DG where VκJκ rearrangements were first recovered. The VλJλ rearrangements recovered at 20-50 DG used some of the same Vλ and Jλ segments seen in older fetuses and adult animals. Hence the textbook paradigm for the order of light-chain rearrangement does not apply to swine. Consistent with weak Tdt expression in early sites of lymphogenesis, N-region additions in VDJ rearrangements were more frequent at 95 DG. Junctional diversity in VλJλ rearrangement was limited at all stages of development. There was little evidence for B-cell lymphogenesis in the ileal Peyer's patches. The widespread recovery of VpreB transcripts in whole, non-lymphoid tissue was unexpected as was its recovery from bone marrow and peripheral blood monocytes. Based on recovery of SJC, B-cell lymphogenesis continues for at least 5 weeks postpartum. |
Databáze: | OpenAIRE |
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