Injectable Alginate Hydrogel Cross-Linked by Calcium Gluconate-Loaded Porous Microspheres for Cartilage Tissue Engineering
| Autor: | Yi-Xing Huang, Ying Qu, Wang Beiyu, Jinrong Peng, Zhiyong Qian, Jinfeng Liao |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Scaffold Materials science General Chemical Engineering chemistry.chemical_element 02 engineering and technology Calcium Cartilage tissue engineering Article lcsh:Chemistry 03 medical and health sciences chemistry.chemical_compound Porous microspheres medicine Cartilage General Chemistry 021001 nanoscience & nanotechnology 030104 developmental biology medicine.anatomical_structure lcsh:QD1-999 chemistry Implant Alginate hydrogel 0210 nano-technology Ethylene glycol Biomedical engineering |
| Zdroj: | ACS Omega ACS Omega, Vol 2, Iss 2, Pp 443-454 (2017) |
| ISSN: | 2470-1343 |
| Popis: | A great interest has been shown in the injectable scaffolds for cartilage tissue regeneration because it can fill irregularly shaped defects easily through minimally invasive surgical treatments. Herein, we developed a new injectable three-dimensional (3D) alginate hydrogel loaded with biodegradable porous poly(ε-caprolactone)–b-poly(ethylene glycol)–b-poly(ε-caprolactone) microspheres (MPs/Alg) as the calcium gluconate container to cross-link alginate. Suspensions of chondrocytes/alginate and porous microspheres turned into a gel because of the release of calcium gluconate; thus, the injectable composite hydrogels give a 3D scaffold to fit the defects perfectly and integrate the extracellular-matrix-mimicking architecture to efficiently accommodate cartilage cells in situ. Tissue repair in a full-thickness cartilage defect model was controlled at 6, 12, and 18 weeks after the implant by micro-CT and immunohistochemistry to evaluate the healing status. The defect in the MPs/Alg+ cells group achieved an almost complete repair at 18 weeks, and the repaired chondrocytes regained a normal tissue structure. Moreover, the MPs/Alg+ cells-treated group increased the quality of tissue formed, including the accumulated glycosaminoglycan and the uniformly deposited type II collagen. The results point out the promising application of the injectable MPs/Alg-chondrocytes system for cartilage tissue engineering. |
| Databáze: | OpenAIRE |
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