The brain is required for normal muscle and nerve patterning during early Xenopus development
| Autor: | Vaibhav P. Pai, Michael Levin, Joan M. Lemire, Celia Herrera-Rincon, Kristine M. Moran |
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| Jazyk: | angličtina |
| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Nervous system medicine.medical_specialty Embryo Nonmammalian Body Patterning Science Xenopus Morphogenesis General Physics and Astronomy In situ hybridization Xenopus Proteins Nervous System General Biochemistry Genetics and Molecular Biology Article 03 medical and health sciences Xenopus laevis 0302 clinical medicine Internal medicine Muscarinic acetylcholine receptor medicine Hyperpolarization-Activated Cyclic Nucleotide-Gated Channels Animals lcsh:Science In Situ Hybridization Multidisciplinary biology Muscles Embryogenesis Brain Gene Expression Regulation Developmental Embryo General Chemistry biology.organism_classification Cell biology 030104 developmental biology Endocrinology medicine.anatomical_structure lcsh:Q 030217 neurology & neurosurgery Signal Transduction |
| Zdroj: | Nature Communications, Vol 8, Iss 1, Pp 1-18 (2017) Nature Communications |
| ISSN: | 2041-1723 |
| DOI: | 10.1038/s41467-017-00597-2 |
| Popis: | Possible roles of brain-derived signals in the regulation of embryogenesis are unknown. Here we use an amputation assay in Xenopus laevis to show that absence of brain alters subsequent muscle and peripheral nerve patterning during early development. The muscle phenotype can be rescued by an antagonist of muscarinic acetylcholine receptors. The observed defects occur at considerable distances from the head, suggesting that the brain provides long-range cues for other tissue systems during development. The presence of brain also protects embryos from otherwise-teratogenic agents. Overexpression of a hyperpolarization-activated cyclic nucleotide-gated ion channel rescues the muscle phenotype and the neural mispatterning that occur in brainless embryos, even when expressed far from the muscle or neural cells that mispattern. We identify a previously undescribed developmental role for the brain and reveal a non-local input into the control of early morphogenesis that is mediated by neurotransmitters and ion channel activity. Functions of the embryonic brain prior to regulating behavior are unclear. Here, the authors use an amputation assay in Xenopus laevis to demonstrate that removal of the brain early in development alters muscle and peripheral nerve patterning, which can be rescued by modulating bioelectric signals. |
| Databáze: | OpenAIRE |
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