Enhanced resolution and coherence lifetimes in the solid-state NMR spectroscopy of perdeuterated proteins under ultrafast magic-angle spinning
| Autor: | Lyndon Emsley, Józef R. Lewandowski, Sascha Lange, Hartmut Oschkinat, Uemit Akbey, Jean-Nicolas Dumez |
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| Přispěvatelé: | ISA - Centre de RMN à très hauts champs, Institut des Sciences Analytiques (ISA), Institut de Chimie du CNRS (INC)-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Centre National de la Recherche Scientifique (CNRS)-Institut de Chimie du CNRS (INC)-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Centre National de la Recherche Scientifique (CNRS), Leibniz Forschungsinstitut für Molekulare Pharmakolgie = Leibniz Institute for Molecular Pharmacology [Berlin, Allemagne] (FMP), Leibniz Association, Agence Nationale de la Recherche (ANR PCV 2007 Protein Motion), DFG (SFB 740), Access to Research Infrastructures activity in the seventh Framework Program of the EC (BioNMR 261863), EU IRG (PIRG03-GA-2008-231026)., European Project, Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS)-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS) |
| Jazyk: | angličtina |
| Rok vydání: | 2011 |
| Předmět: |
Analytical chemistry
ASSIGNMENT Field strength 010402 general chemistry 01 natural sciences LATTICE-RELAXATION [CHIM.ANAL]Chemical Sciences/Analytical chemistry Magic angle spinning General Materials Science RATES Physical and Theoretical Chemistry Spectroscopy Spinning 010405 organic chemistry Chemistry H-1 CRYSTALLINE PROTEIN DEUTERATION QUANTITATIVE-ANALYSIS 0104 chemical sciences NMR spectra database [CHIM.THEO]Chemical Sciences/Theoretical and/or physical chemistry Microcrystalline Solid-state nuclear magnetic resonance ROTATING SOLIDS BACKBONE DYNAMICS [PHYS.PHYS.PHYS-CHEM-PH]Physics [physics]/Physics [physics]/Chemical Physics [physics.chem-ph] SENSITIVITY Coherence (physics) |
| Zdroj: | Journal of Physical Chemistry Letters Journal of Physical Chemistry Letters, American Chemical Society, 2011, 2 (17), pp.2205-2211. ⟨10.1021/jz200844n⟩ Journal of Physical Chemistry Letters, 2011, 2 (17), pp.2205-2211. ⟨10.1021/jz200844n⟩ |
| ISSN: | 1948-7185 |
| DOI: | 10.1021/jz200844n⟩ |
| Popis: | International audience; We investigate the combined effect of perdeuteration and fast magic-angle spinning on the resolution and sensitivity of proton-detected protein NMR spectra and on coherence lifetimes. With 60 kHz spinning of a microcrystalline alpha-spectrin SH3 sample at a field strength of 23 T, a regime is attained where there is no substantial difference in resolution between perdeuterated samples with 10 or 100% protons at the exchangeable sites. (1)H resolution is then limited by inhomogeneous contributions. Upon fast spinning, the most dramatic line narrowing effects are observed for residues in the loop or bend regions of the protein, probably due to the removal of destructive dynamics effects. This investigation paves the way for using samples with 100% protons at the exchangeable sites in structure determination protocols, since all backbone amide sites can now contribute to the signal. |
| Databáze: | OpenAIRE |
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