Slit1 promotes regenerative neurite outgrowth of adult dorsal root ganglion neurons in vitro via binding to the Robo receptor
| Autor: | Xi Nan Yi, Zhi Bin Chen, Linfeng Zheng, Dan Zhao, Hai Ying Zhang, Zhi Jian Ma, Xian Fang Zhang, Zhong Ping He |
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| Rok vydání: | 2009 |
| Předmět: |
Male
Neurite Sensory Receptor Cells Neurogenesis Recombinant Fusion Proteins Growth Cones Nerve Tissue Proteins Rats Sprague-Dawley Cellular and Molecular Neuroscience Organ Culture Techniques Dorsal root ganglion Ganglia Spinal SLIT1 medicine Neurites Animals Receptors Immunologic Receptor Messenger RNA Chemistry Cell migration Cell Differentiation Slit Cell biology Nerve Regeneration Rats medicine.anatomical_structure nervous system Axon guidance Neuroscience |
| Zdroj: | Journal of chemical neuroanatomy. 39(4) |
| ISSN: | 1873-6300 |
| Popis: | Secreted Slit proteins have previously been shown to signal through Roundabout (Robo) receptors to negatively regulate axon guidance and cell migration. During vertebrate development, Slit proteins have also been shown to stimulate branching and elongation of sensory axons and cortical dendrites. In this study, Slit1/Robo2 mRNA and protein expressions were detected in adult rat dorsal root ganglion (DRG) and in cultured DRG neurons. Treatment of both models with recombinant, soluble Slit1 protein was found to promote neurite outgrowth and elongation. In contrast, treatment with a recombinant human Robo2/Fc chimera inhibited neurite outgrowth and elongation. When adult DRG and cultured DRG neurons were pretreated with soluble recombinant human Robo2/Fc chimera, neurite outgrowth and elongation was not induced. These findings indicate that Slit1/Robo2 signaling may have a role in regulating peripheral nerve regeneration. |
| Databáze: | OpenAIRE |
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