Roles for BTAF1 and Mot1p in dynamics of TATA-binding protein and regulation of RNA polymerase II transcription
| Autor: | Marcin P. Klejman, H. Th. Marc Timmers, Lloyd Ashley Pereira |
|---|---|
| Rok vydání: | 2003 |
| Předmět: |
Saccharomyces cerevisiae Proteins
Transcription Genetic RNA polymerase II Models Biological Genetics Animals Humans RNA polymerase II holoenzyme Adenosine Triphosphatases TATA-Binding Protein Associated Factors biology DNA Helicases General Medicine TATA-Box Binding Protein Cell biology biology.protein Transcription factor II F Transcription Factor TFIID Transcription factor II E RNA Polymerase II TATA-binding protein Transcription factor II D Transcription factor II B Transcription factor II A Protein Binding |
| Zdroj: | Gene. 315 |
| ISSN: | 0378-1119 |
| Popis: | Regulation of RNA polymerase II (pol II) transcription is a highly dynamic process requiring the coordinated interaction of an array of regulatory proteins. Central to this process is the TATA-binding protein (TBP), the key component of the multiprotein complex TFIID. Interaction of TBP with core promoters nucleates the assembly of the preinitiation complex and subsequent recruitment of pol II. Despite recent advances in our understanding of the dynamic nature of the pol II transcription apparatus, the dynamics of TBP function on pol II promoters has remained largely unexplored. Human BTAF1 (TAF(II)170/TAF-172) and its yeast ortholog, Mot1p, are evolutionarily conserved members of the SNF2-like family of ATPase proteins. Genetic identification of Mot1p as a repressor of pol II transcription was supported by findings that Mot1p and BTAF1 could dissociate TBP from TATA DNA complexes using the energy of ATP hydrolysis. Recent data have revealed new aspects of BTAF1 and Mot1p as positive regulators of TBP function in the pol II system and have described new observations relating to their molecular mechanism of action. We review these data in the context of previous findings with particular attention paid to how human BTAF1 and Mot1p may dynamically regulate TBP function on pol II promoters in cells. |
| Databáze: | OpenAIRE |
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