Multiplexed Molecular Imaging Strategy Integrated with RNA Sequencing in the Assessment of the Therapeutic Effect of Wharton’s Jelly Mesenchymal Stem Cell-Derived Extracellular Vesicles for Osteoporosis
| Autor: | Fong-Shya Jeng, Yi-An Chen, Cheng-Hsiu Lu, Sain-Jhih Chiu, Ren Shyan Liu, Chi-Wei Chang, Chien-Chih Ke, Ya-Ju Hsieh, Feng-Sheng Wang, Bang-Hung Yang, Chao-Cheng Chen |
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| Rok vydání: | 2021 |
| Předmět: |
[18F]NaF
Biophysics Pharmaceutical Science Bioengineering Extracellular vesicles Biomaterials Mice Phosphatidylinositol 3-Kinases International Journal of Nanomedicine Drug Discovery Wharton's jelly Animals Wharton Jelly Original Research Sequence Analysis RNA Chemistry Organic Chemistry Therapeutic effect Mesenchymal stem cell RNA Mesenchymal Stem Cells RNA sequencing General Medicine osteoporosis Wharton’s jelly mesenchymal stem cells Molecular Imaging Cell biology MicroRNAs Molecular imaging extracellular vesicles |
| Zdroj: | International Journal of Nanomedicine |
| ISSN: | 1178-2013 |
| DOI: | 10.2147/ijn.s335757 |
| Popis: | Introduction Osteoporosis is a result of an imbalance in bone remodeling. Mesenchymal stem cell-derived extracellular vesicles (MSC-EVs) have been considered as a potentially promising treatment for osteoporosis. However, the therapeutic effect, genetic alterations, and in vivo behavior of exogenous EVs for osteoporosis in mice models remain poorly understood. Methods A multiplexed molecular imaging strategy was constructed by micro-positron emission tomography (µPET)/computed tomography (CT), µCT, and optical imaging modality which reflected the osteoblastic activity, microstructure, and in vivo behavior of EVs, respectively. RNA sequencing was used to analyze the cargo of EVs, and the bone tissues of ovariectomized (OVX) mice post EV treatment. Results The result of [18F]NaF µPET showed an increase in osteoblastic activity in the distal femur of EV-treated mice, and the bone structural parameters derived from µCT were also improved. In terms of in vivo behavior of exogenous EVs, fluorescent dye-labeled EVs could target the distal femur of mice, whereas the uptakes of bone tissues were not significantly different between OVX mice and healthy mice. RNA sequencing demonstrated upregulation of ECM-related genes, which might associate with the PI3K/AKT signaling pathway, in line with the results of microRNA analysis showing that mir-21, mir-29, mir-221, and let-7a were enriched in Wharton’s jelly-MSC-EVs and correlated to the BMP and PI3K/AKT signaling pathways. Conclusion The therapeutic effect of exogenous WJ-MSC-EVs in the treatment of osteoporosis was successfully assessed by a multiplexed molecular imaging strategy. The RNA sequencing demonstrated the possible molecular targets in the regulation of bone remodeling. The results highlight the novelty of diagnostic and therapeutic strategies of EV-based treatment for osteoporosis. Graphical Abstract |
| Databáze: | OpenAIRE |
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