Cyclic pulsation stress promotes bone formation of tissue engineered laminae through the F-actin/YAP-1/β-Catenin signaling axis
| Autor: | Li, Linli, Li, Hailong, He, Yiqun, Tang, Han, Dong, Jian, Chen, Xujun, Lyu, Feizhou, Dong, Youhai |
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| Rok vydání: | 2021 |
| Předmět: |
YAP1
Chemistry Angiogenesis Regeneration (biology) Mesenchymal stem cell Biomedical Engineering Medicine (miscellaneous) Cell Biology Article Stem-cell biotechnology Cell biology Cerebrospinal fluid medicine.anatomical_structure Checkpoint signalling medicine Mesenchymal stem cells Medicine Mechanotransduction Actin Developmental Biology Blood vessel |
| Zdroj: | npj Regenerative Medicine, Vol 6, Iss 1, Pp 1-13 (2021) NPJ Regenerative Medicine |
| ISSN: | 2057-3995 |
| DOI: | 10.1038/s41536-021-00164-w |
| Popis: | Mechanical loads are fundamental regulators of bone formation and remodeling. However, the molecular regulation of mechanotransduction during vertebral laminae regeneration remains poorly understood. Here, we found that cerebrospinal fluid pulsation (CSFP) stress—cyclic pulsation stress—could promote the osteogenic and angiogenic abilities of rat mesenchymal stromal cells (MSC), thereby promoting tissue-engineered laminae’s bone and blood vessel formation. In the process, F-actin relayed CSFP stress to promote the nuclear translocation of YAP1, which then decreased the degradation and promoted the nuclear translocation of β-Catenin. In turn, the nuclear translocation of β-Catenin promoted the osteogenic differentiation and angiogenic abilities of MSC, thereby promoting tissue-engineered laminae’s bone and blood vessel formation. Thus, we conclude that CSFP promotes the osteogenesis and angiogenesis of tissue-engineered laminae through the F-actin/YAP-1/β-Catenin signaling axis. This study advances our understanding of vertebral laminae regeneration and provides potential therapeutic approaches for spinal degeneration after spinal laminectomy. |
| Databáze: | OpenAIRE |
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