Endovascular Interventions Permit Isolation of Endothelial Colony-Forming Cells from Peripheral Blood

Autor: Mariam Yu. Khanova, V. A. Matveeva, Egor S. Sardin, Larisa V. Antonova, Olga Barbarash
Jazyk: angličtina
Rok vydání: 2018
Předmět:
0301 basic medicine
medicine.medical_treatment
Cell Separation
030204 cardiovascular system & hematology
lcsh:Chemistry
Coronary artery disease
endothelial colony-forming cells
Coronary artery bypass surgery
0302 clinical medicine
lcsh:QH301-705.5
Spectroscopy
Cells
Cultured
Endothelial Progenitor Cells
coronary artery bypass surgery
Catheter insertion
Endovascular Procedures
General Medicine
Middle Aged
peripheral blood mononuclear cells
Computer Science Applications
medicine.anatomical_structure
Phenotype
surgical procedures
operative
Cardiology
Artery
medicine.medical_specialty
Neovascularization
Physiologic
Peripheral blood mononuclear cell
Catalysis
Article
Inorganic Chemistry
Colony-Forming Units Assay
03 medical and health sciences
Internal medicine
Angioplasty
medicine
Human Umbilical Vein Endothelial Cells
Humans
cardiovascular diseases
Physical and Theoretical Chemistry
Antigens
Molecular Biology
Aged
Cell Proliferation
business.industry
Organic Chemistry
percutaneous coronary intervention
Percutaneous coronary intervention
medicine.disease
030104 developmental biology
lcsh:Biology (General)
lcsh:QD1-999
Conventional PCI
Leukocytes
Mononuclear
proliferative activity
Leukocyte Common Antigens
business
Zdroj: International Journal of Molecular Sciences
Volume 19
Issue 11
International Journal of Molecular Sciences, Vol 19, Iss 11, p 3453 (2018)
ISSN: 1422-0067
DOI: 10.3390/ijms19113453
Popis: Background: Isolation of endothelial colony-forming cells (ECFCs) is difficult due to the extremely low concentration of their precursors in the peripheral blood (PB). We hypothesized that mechanical injury to the arterial wall during percutaneous coronary intervention (PCI) or coronary artery bypass grafting (CABG) may increase the release of circulating ECFC precursors and induce their growth in vitro. Methods: PB samples from patients with coronary artery disease were collected before, immediately after, and 24 h after the surgery in the CABG group. In the PCI group, PB was isolated before, immediately after the insertion of the catheter, immediately after balloon angioplasty, and 24 h after the PCI. A mononuclear fraction of PB was isolated and differentiated into ECFCs with the following immunophenotyping and evaluation of angiogenic properties. Results. The obtained cultures corresponded to the phenotype and tube forming potential consistent with ECFCs. The isolation of ECFCs in the PCI group was successful in 75% of cases (six out of eight patients) after catheter insertion and in 87.5% (seven out of eight patients) after the balloon inflation and stent deployment. These cultures had high/medium proliferative activity in contrast to those obtained before or 24 h after the intervention. Conclusions: Mechanical injury during PCI increases the release of ECFC precursors to the PB and, hence, the efficacy of ECFC isolation.
Databáze: OpenAIRE
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