Autoradiographic studies of chromosome replication during the cell cycle of Streptococcus faecium
| Autor: | Michael L. Higgins, A L Koch, D T Dicker, Lolita Daneo-Moore |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 1986 |
| Předmět: |
DNA Replication
DNA Bacterial Cell Population Biology Microbiology chemistry.chemical_compound Replication (statistics) medicine Doubling time education Molecular Biology Genetics education.field_of_study Cell Cycle DNA replication Chromosome Streptococcus Cell cycle Chromosomes Bacterial Molecular biology medicine.anatomical_structure chemistry Autoradiography Thymidine Research Article |
| Popis: | Analysis of the distribution of autoradiographic grains around cells of Streptococcus faecium which had been either continuously or pulse-labeled with tritiated thymidine (mass doubling time, 90 min) showed a non-Poisson distribution even when the distribution of cell sizes in the populations studied was taken into account. These non-Poisson distributions of grains were assumed to reflect the discontinuous nature of chromosome replication. To study this discontinuous process further, we fitted an equation to the grain distribution observed for the pulse-labeled cells that assumed that in any population of cells there were subpopulations in which there were zero, one, or two replicating chromosomes. This analysis predicted an average time for chromosome replication and for the period between completion of rounds of chromosome replication and division of 55 and 43 min, respectively, which were in excellent agreement with estimates made by other techniques. The present investigation extended past studies in indicating that the initiation and completion of rounds of chromosome replication are poorly phased with increases in cell volume and that the amount of chromosome replication may be different in different cell halves. |
| Databáze: | OpenAIRE |
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