Effects of chondrogenic and osteogenic regulatory factors on composite constructs grown using human mesenchymal stem cells, silk scaffolds and bioreactors
Autor: | Nipun Patel, Michelle Farley, Charu Vepari, David L. Kaplan, Martha L. Gray, Lisa E. Freed, Alexander Augst, Lorenz Meinel, Robert Fajardo, Gordana Vunjak-Novakovic, Darja Marolt |
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Rok vydání: | 2008 |
Předmět: |
Cell Culture Techniques
Silk Biomedical Engineering Biophysics Bone Morphogenetic Protein 2 Bioengineering Ascorbic Acid Bone morphogenetic protein Biochemistry Bone morphogenetic protein 2 Dexamethasone Transforming Growth Factor beta1 Biomaterials Bioreactors Tissue engineering Osteogenesis Transforming Growth Factor beta medicine Humans Insulin Analysis of Variance Tissue Engineering Chemistry Cartilage Mesenchymal stem cell Mesenchymal Stem Cells Anatomy equipment and supplies Chondrogenesis Ascorbic acid Immunohistochemistry Magnetic Resonance Imaging Cell biology medicine.anatomical_structure Glycerophosphates Bone Morphogenetic Proteins Bone marrow Research Article Biotechnology |
Zdroj: | Journal of The Royal Society Interface. 5:929-939 |
ISSN: | 1742-5662 1742-5689 |
DOI: | 10.1098/rsif.2007.1302 |
Popis: | Human mesenchymal stem cells (hMSCs) isolated from bone marrow aspirates were cultured on silk scaffolds in rotating bioreactors for three weeks with either chondrogenic or osteogenic medium supplements to engineer cartilage- or bone-like tissue constructs. Osteochondral composites formed from these cartilage and bone constructs were cultured for an additional three weeks in culture medium that was supplemented with chondrogenic factors, supplemented with osteogenic factors or unsupplemented. Progression of cartilage and bone formation and the integration between the two regions were assessed by medical imaging (magnetic resonance imaging and micro-computerized tomography imaging), and by biochemical, histological and mechanical assays. During composite culture (three to six weeks), bone-like tissue formation progressed in all three media to a markedly larger extent than cartilage-like tissue formation. The integration of the constructs was most enhanced in composites cultured in chondrogenic medium. The results suggest that tissue composites with well-mineralized regions and substantially less developed cartilage regions can be generated in vitro by culturing hMSCs on silk scaffolds in bioreactors, that hMSCs have markedly higher capacity for producing engineered bone than engineered cartilage, and that chondrogenic factors play major roles at early stages of bone formation by hMSCs and in the integration of the two tissue constructs into a tissue composite. |
Databáze: | OpenAIRE |
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