LOH analysis of genes around D4S2964 identifies ARD1B as a prognostic predictor of hepatocellular carcinoma
| Autor: | Mei Yin Zhang, Yunfei Yuan, An Hua Li, Bi Jun Huang, Ming Shi, Xiao Qian Chen, Bin Kui Li, Guo Liang Huang, Hui Zhong Zhang, Hui Yun Wang, Hong Hua Li, Rong Rong Wei, Long Huang |
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| Rok vydání: | 2010 |
| Předmět: |
Adult
Male Carcinoma Hepatocellular Adolescent Brief Article Microarray Molecular Sequence Data Loss of Heterozygosity Cell Cycle Proteins Locus (genetics) Single-nucleotide polymorphism Biology Polymorphism Single Nucleotide Loss of heterozygosity Acetyltransferases medicine Carcinoma Humans neoplasms Survival rate N-Terminal Acetyltransferase A Aged Oligonucleotide Array Sequence Analysis Receptors Scavenger Base Sequence ADP-Ribosylation Factors Liver Neoplasms Gastroenterology Lysosome-Associated Membrane Glycoproteins General Medicine Middle Aged Nucleoporin 54 medicine.disease Fibrosis Molecular biology digestive system diseases stomatognathic diseases Hepatocellular carcinoma Cancer research Female Septins |
| Zdroj: | World Journal of Gastroenterology. 16:2046 |
| ISSN: | 1007-9327 |
| Popis: | AIM: To investigate genes around the locus D4S2964 affected by loss of heterozygosity (LOH) and their clinical implications. METHODS: Four hundred and forty single nucleotide polymorphisms (SNPs) located at 49 genes around D4S2964 were selected from the National Center for Biotechnology Information website for the SNPs microarray fabrication. LOH of SNPs markers in 112 cases of hepatocellular carcinoma (HCC) tissues and paired adjacent liver tissues were investigated by the SNPs microarray. The correlation between allelic losses with clinicopathological features and overall survival was analyzed. RESULTS: A fine map of LOH of SNPs in genes around D4S2964 was plotted. The average frequency of LOH in genes was 0.39. A correlation between cirrhosis and the FAL index (fractional allelic loss) was found (P = 0.0202). Larger tumor size was found to be significantly associated with LOH in genes ADP-ribosyltransferase 3 (ART3), nucleoporin 54 kDa (NUP54), scavenger receptor class B, member 2 (SCARB2) and coiled-coil domain containing 158 (CCDC158) (P = 0.043, P = 0.019, P = 0.001, P = 0.037, respectively). Kaplan-Meier analysis showed that patients with LOH in ARD1 homolog B (ARD1B) and septin 11 (SEPT11) had a significantly lower survival rate than those with retention (P = 0.021 and P = 0.004, respectively). A Cox regression model suggested that LOH in ARD1B and SEPT11, respectively, were predictors of the overall survival in HCC (P = 0.006 and P = 0.026, respectively). CONCLUSION: LOH in genes around D4S2964 may play an important role in HCC development and progression. LOH in ARD1B and SEPT11 could serve as novel prognostic predictors in HCC patients. |
| Databáze: | OpenAIRE |
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