Prevalence of RET/PTC rearrangement in benign and malignant thyroid nodules and its clinical application
| Autor: | Maurilio Deandrea, Anna Guerra, Manuela Motta, M. I. Moretti, Mario Vitale, Guido Rossi, Gianfranco Fenzi, Vincenzo Marotta, Maria Rosaria Sapio, Paolo Piero Limone, Elisabetta Campanile |
|---|---|
| Rok vydání: | 2011 |
| Předmět: |
Male
Thyroid nodules endocrine system Pathology medicine.medical_specialty endocrine system diseases Endocrinology Diabetes and Metabolism Biopsy Fine-Needle Sensitivity and Specificity Thyroid carcinoma Endocrinology Humans Medicine False Positive Reactions Thyroid Neoplasms Thyroid Nodule False Negative Reactions Thyroid cancer Aged Tumor marker Gene Rearrangement RET/PTC Rearrangement Reverse Transcriptase Polymerase Chain Reaction business.industry Carcinoma Proto-Oncogene Proteins c-ret Thyroid Cancer Middle Aged medicine.disease Carcinoma Papillary medicine.anatomical_structure Thyroid Cancer Papillary Female Histopathology business |
| Zdroj: | Endocrine Journal. 58:31-38 |
| ISSN: | 1348-4540 0918-8959 |
| DOI: | 10.1507/endocrj.k10e-260 |
| Popis: | Fine-needle aspiration cytology (FNAC) is the primary means to distinguish benign thyroid nodules from malignant ones. About 20% of FNAC yields indeterminate results leading to unnecessary or delayed surgery. Many studies of tissue samples, the majority of which are retrospective advocate testing for RET rearrangements as a diagnostic adjunctive tool in thyroid nodules with indeterminate cytological findings. Because of the uncertain prevalence of RET rearrangements, its utility as a tumor marker is still controversial. The goal of this study was to establish the prevalence and the utility of testing for RET rearrangements in FNAC suspicious of cancer in a clinical setting. In this prospective study, we analysed a large series of thyroid aspirates by RT-PCR only and Southern blot on RT-PCR products for type 1 and 3 RET rearrangements. Results were compared with clinical findings, cytological diagnosis and final histopathology. By the higher sensitive Southern-blot on RT-PCR method, RET rearrangements were present in 36% of papillary thyroid carcinomas (RET/PTC-1, 12%; RET/PTC-3, 20%; both, 4%) and of 13.3% of benign nodules. By means of RT-PCR only, RET rearrangements were disclosed only in 14.3% of PTC and in 3.6% of benign nodules. No significant correlation was found between RET rearrangements and clinicopathological features of patients. These results indicate that molecular testing of thyroid nodules for RET/PTC must take into account of its high prevalence in benign nodules, inducing false positive diagnoses when the highly sensitive assay Southern-blot on RT-PCR is used. Its searching by means of RT-PCR only, has a specificity superior of conventional cytology and can be used to refine inconclusive FNAC. |
| Databáze: | OpenAIRE |
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