Aqueous leaf extract ofMoringa oleiferareduced intracellular ROS production, DNA fragmentation and acrosome reaction in Human spermatozoa in vitro
| Autor: | Gbenga Anthony Adefolaju, Ralf Henkel, Faith T Moichela, Chinyerum S. Opuwari |
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| Rok vydání: | 2020 |
| Předmět: |
Male
Antioxidant Urology medicine.medical_treatment Acrosome reaction 030232 urology & nephrology Semen DNA Fragmentation Andrology 03 medical and health sciences 0302 clinical medicine Endocrinology Capacitation medicine Humans Sperm motility Moringa oleifera chemistry.chemical_classification Reactive oxygen species 030219 obstetrics & reproductive medicine Plant Extracts urogenital system Chemistry Acrosome Reaction General Medicine Spermatozoa Sperm Sperm Motility DNA fragmentation Reactive Oxygen Species Acrosome Sperm Capacitation |
| Zdroj: | Andrologia. 53 |
| ISSN: | 1439-0272 0303-4569 |
| DOI: | 10.1111/and.13903 |
| Popis: | The effects of aqueous leaf extract of Moringa oleifera (MO) on human sperm functions and integrity was studied in vitro. Semen was obtained by masturbation after 3-5 days' abstinence from 34 healthy donors in Western Cape, South Africa. Liquefied semen was washed in human tubular fluid supplemented with 1% bovine serum albumin (HTF-BSA;1:5) with 10 min centrifugation at 300 g. Sperm suspensions were subsequently incubated with MO extract (0.625, 6.25, 62.5 and 625 µg/ml) for 1 hr, where HTF-BSA served as control. Sperm motility, vitality, DNA fragmentation, reactive oxygen species production, mitochondrial membrane potential, capacitation and acrosome reaction were assessed. Sperm motility, vitality, mitochondrial membrane potential and capacitation remained unchanged (p > .05). A dose-dependent decrease in sperm reactive oxygen species production (p < .0001), DNA fragmentation (p < .0001) and acrosome reaction (p < .001) was observed. An increase in the percentage of non-capacitated sperm (p < .01) was noted at 625 µg/ml. The antioxidant properties of MO actively maintained basic sperm functions, inhibited excess sperm free superoxide production and preserved acrosome reaction and DNA integrity. Further studies are needed to confirm the effect of aqueous MO leaf extract on fertility potential. |
| Databáze: | OpenAIRE |
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