Foliar CO2 fixation in bean (Phaseolus vulgaris L.) submitted to elevated ozone: distinct changes in Rubisco and PEPc activities in relation to pigment content
| Autor: | Jean-Philippe Biolley, Louis Leitao, Pierre Dizengremel |
|---|---|
| Přispěvatelé: | Université de Pau et des Pays de l'Adour (UPPA), Ecologie et Ecophysiologie Forestières [devient SILVA en 2018] (EEF), Institut National de la Recherche Agronomique (INRA)-Université de Lorraine (UL) |
| Jazyk: | angličtina |
| Rok vydání: | 2008 |
| Předmět: |
0106 biological sciences
inorganic chemicals Oxygenase PHOSPHOENOLPYRUVATE CARBOXYLASE Health Toxicology and Mutagenesis Ribulose-Bisphosphate Carboxylase [SDV]Life Sciences [q-bio] PEPC BEAN Oxidative phosphorylation 01 natural sciences 03 medical and health sciences Pigment Botany 030304 developmental biology Phaseolus 0303 health sciences biology OZONE Carbon fixation RuBisCO fungi Public Health Environmental and Occupational Health food and beverages General Medicine Carbon Dioxide PIGMENTS biology.organism_classification PROTEIN CARBONYLS HARICOT Pollution RUBISCO Pyruvate carboxylase Oxygen Plant Leaves visual_art visual_art.visual_art_medium biology.protein RIBULOSE-1 Seasons Phosphoenolpyruvate carboxylase 5-BIPHOSPHATE CARBOXYLASE/OXYGENASE 010606 plant biology & botany |
| Zdroj: | Ecotoxicology and Environmental Safety Ecotoxicology and Environmental Safety, Elsevier, 2008, 69 (3), pp.531-540. ⟨10.1016/j.ecoenv.2006.10.010⟩ |
| ISSN: | 0147-6513 1090-2414 |
| DOI: | 10.1016/j.ecoenv.2006.10.010⟩ |
| Popis: | Using open-top chambers, the impact of ozone (O3) on foliar carboxylases of bean (Phaseolus vulgaris L.) was investigated. From sowing, beans were exposed to non-filtered air (NF) and NF supplied with 40 (+40) and 80 (+80) nL L−1 O3. Twenty days after emergence, primary and first trifoliate leaves were sampled. Biochemical characteristics of leaves from +40 were quite similar to those from NF. Conversely, +80 induced distinct biochemical effects in primary and first trifoliate leaves. Regarding primary leaves, +80 reduced ribulose-1,5-bisphosphate carboxylase/oxygenase (Rubisco) activity by 33% whereas it stimulated phosphoenolpyruvate carboxylase (PEPc) activity by 376%. The reduction in Rubisco activity was accompanied by a decrease in both Rubisco subunit amounts and a consistent oxidative modification of the Rubisco small subunit (SSU). These changes came with a drastic loss in pigmentation. Regarding first trifoliate leaves, +80 stimulated Rubisco activity by 33% while it disturbed neither PEPc activity nor pigmentation. Surprisingly, the enhanced Rubisco activity was associated with a slight decrease in Rubisco protein quantity, which was not coupled with the formation of carbonyl groups in Rubisco-SSU. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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