Adipose-specific BMP and activin membrane-bound inhibitor (BAMBI) deletion promotes adipogenesis by accelerating ROS production
| Autor: | Yanting Xu, Taiyong Yu, Gongshe Yang, Xiaoge Zhou, Xiaochang Chen, Kuilong Huang, Xiaoyu Wang, Chen Zhao, Weijun Pang, Yulong Wang |
|---|---|
| Rok vydání: | 2021 |
| Předmět: |
0301 basic medicine
HFD high-fat diet OXPHOS oxidative phosphorylation NAC N-acetylcysteine Adipose tissue RER respiratory exchange rate medicine.disease_cause Biochemistry Mice chemistry.chemical_compound insulin resistance LDL low-density lipoprotein Adipocyte GM growth medium Brown adipose tissue DMEM Dulbecco’s modified Eagle medium ROS production TGF transforming growth factor Mice Knockout NOX4 Cell Differentiation TG triglycerides NOX NADPH oxidase Cell biology TNFα tumor necrosis factor α medicine.anatomical_structure Adipose Tissue Adipogenesis Bone Morphogenetic Proteins Research Article HDL high-density lipoprotein Diet High-Fat Bone morphogenetic protein MCP1 monocyte chemoattractant protein 1 adipogenesis 03 medical and health sciences ROS reactive oxygen species MCE mitotic clonal expansion medicine Animals Humans Molecular Biology BAMBI 030102 biochemistry & molecular biology CCAAT-Enhancer-Binding Protein-beta NADPH oxidase 4 Membrane Proteins BAMBI bone morphogenetic protein and activin membrane-bound inhibitor RT-qPCR real-time quantitative PCR Cell Biology NADPH nicotinamide adenine dinucleotide phosphate Acetylcysteine BAT brown adipose tissue Fatty Liver 030104 developmental biology chemistry CD chow diet H&E hematoxylin and eosin Reactive Oxygen Species Oxidative stress |
| Zdroj: | The Journal of Biological Chemistry |
| ISSN: | 0021-9258 |
| DOI: | 10.1074/jbc.ra120.014793 |
| Popis: | With the improvement of people's living standards, the number of obese patients has also grown rapidly. It is reported that the level of oxidative stress in obese patients has significantly increased, mainly caused by the increase in reactive oxygen species (ROS) levels in adipose tissue. Studies have shown that the use of siRNA to interfere with bone morphogenetic protein and activin membrane-bound inhibitor (BAMBI) expression could promote adipocyte differentiation, and under hypoxic conditions, BAMBI could act as a regulator of HIF1α to regulate the polarity damage of epithelial cells. In view of these results, we speculated that BAMBI may regulate adipogenesis by regulating the level of ROS. In this study, we generated adipose-specific BAMBI knockout mice (BAMBI AKO) and found that compared with control mice, BAMBI AKO mice showed obesity when fed with high-fat diet, accompanied by insulin resistance, glucose intolerance, hypercholesterolemia, and increased inflammation in adipose tissue. Interestingly, adipose-specific deficiency of BAMBI could cause an increase in the expression level of Nox4, thereby promoting ROS production in cytoplasm and mitochondria and the DNA-binding activity of C/EBPβ and ultimately promoting adipogenesis. Consistently, our findings indicated that BAMBI may be a reactive oxygen regulator to affect adipogenesis, thereby controlling obesity and metabolic syndrome. |
| Databáze: | OpenAIRE |
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