Use of organic solvent to prevent protein adsorption in CE-MS experiments
| Autor: | Julie Schappler, Serge Rudaz, Aline Staub, Jean-Luc Veuthey, Sylvain Comte |
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| Rok vydání: | 2010 |
| Předmět: |
Acetonitriles
Capillary action Clinical Biochemistry Biochemistry Analytical Chemistry Capillary electrophoresis Hydrophobic effect Adsorption Organic chemistry Molecule Organic Chemicals ddc:615 Ethanol Viscosity Chemistry Methanol Electrophoresis Capillary Proteins Hydrogen-Ion Concentration Electrostatics Solvent Models Chemical Chemical engineering Organic solvent Solvents Electroosmosis Hydrophobic and Hydrophilic Interactions Protein adsorption |
| Zdroj: | Electrophoresis, Vol. 31, No 19 (2010) pp. 3326-3333 |
| ISSN: | 0173-0835 |
| DOI: | 10.1002/elps.201000245 |
| Popis: | Protein adsorption onto capillary wall often hampers CE separations, particularly in the CZE mode. Electrostatic interactions are not the only factors affecting adsorption, as hydrophobic interactions and/or protein conformational changes are also involved in the adsorption phenomenon. Numerous methods can be used to reduce or avoid adsorption, such as (i) addition of low molecular weight molecules in the BGE, (ii) use of surfactants, or (iii) capillary coatings. However, most of these methods are not MS-compatible. In this study, we evaluated the addition of organic solvent as an alternative MS-compatible method to decrease protein adsorption. The effect of the solvent addition was emphasized using classical methods for estimating reversible and irreversible adsorption. In many cases, organic solvents were effective at decreasing adsorption. However, the influence of the organic solvent on protein adsorption should be evaluated case-by-case in CE method development. |
| Databáze: | OpenAIRE |
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