Mouse Lymphoma Thymidine Kinase Gene Mutation Assay: International Workshop on Genotoxicity Tests Workgroup Report—Plymouth, UK 2002
| Autor: | Michael R. O’Donovan, George Bolcsfoldi, Martha M. Moore, Robert Preston, Stephan Kirchner, Michael D. Fellows, Paul Kirby, Richard H.C. San, Ajit K. Thakur, Brian Myhr, Takashi Omori, Isao Yoshimura, Wolfgang Muster, Masamitsu Honma, Maria A. Cifone, B. Bhaskar Gollapudi, Peter Jenkinson, Robert R. Delongchamp, Shinobu Wakuri, Julie Clements, Marie-Claude Ouldelhkim, Colin Riach, Jo Oliver, Kamala Pant, Leon F. Stankowski |
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| Rok vydání: | 2003 |
| Předmět: |
Genetics
medicine.medical_specialty Lymphoma Mutagenicity Tests Thymidine Kinase Gene Health Toxicology and Mutagenesis Mouse Lymphoma Experimental data Positive control Biology medicine.disease_cause Thymidine Kinase Suspension culture Mice Soft agar medicine Animals Biological Assay Medical physics Workgroup Genotoxicity |
| Zdroj: | Mutation Research/Genetic Toxicology and Environmental Mutagenesis. 540:127-140 |
| ISSN: | 1383-5718 |
| Popis: | The Mouse Lymphoma Assay (MLA) Workgroup of the International Workshop on Genotoxicity Tests (IWGT) met on June 28th and 29th, 2002, in Plymouth, England. This meeting of the MLA group was devoted to discussing the criteria for assay acceptance and appropriate approaches to data evaluation. Prior to the meeting, the group conducted an extensive analysis of data from both the microwell and soft agar versions of the assay. For the establishment of criteria for assay acceptance, 10 laboratories (6 using the microwell method and 4 using soft agar) provided data on their background mutant frequencies, plating efficiencies of the negative/vehicle control, cell suspension growth, and positive control mutant frequencies. Using the distribution curves generated from this data, the Workgroup reached consensus on the range of values that should be used to determine whether an individual experiment is acceptable. In order to establish appropriate approaches for data evaluation, the group used a number of statistical methods to evaluate approximately 400 experimental data sets from 10 laboratories entered into a database created for the earlier MLA Workshop held in New Orleans [Environ. Mol. Mutagen. 40 (2002) 292]. While the Workgroup could not, during this meeting, make a final recommendation for the evaluation of data, a general strategy was developed and the Workgroup members agreed to evaluate this new proposed approach using their own laboratory data. This evaluation should lead to a consensus global approach for data evaluation in the near future. |
| Databáze: | OpenAIRE |
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