Measurement of absolute blood flow velocity in outflow tract of HH18 chicken embryo based on 4D reconstruction using spectral domain optical coherence tomography
| Autor: | Sandra Rugonyi, Aiping Liu, Ruikang K. Wang, Kent L. Thornburg, Zhenhe Ma, Xin Yin, Aaron Troyer |
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| Rok vydání: | 2010 |
| Předmět: |
ocis:(110.4155) Multiframe image processing
01 natural sciences 010309 optics 03 medical and health sciences Optics Optical coherence tomography 0103 physical sciences medicine ocis:(170.4500) Optical coherence tomography Image resolution 030304 developmental biology Physics 0303 health sciences medicine.diagnostic_test business.industry Orientation (computer vision) Blood flow Atomic and Molecular Physics and Optics Flow (mathematics) Flow velocity Temporal resolution Outflow Optical Coherence Tomography ocis:(170.3880) Medical and biological imaging business Biotechnology |
| Zdroj: | Biomedical Optics Express |
| ISSN: | 2156-7085 |
| Popis: | The measurement of blood-plasma absolute velocity distributions with high spatial and temporal resolution in vivo is important for the investigation of embryonic heart at its early stage of development. We introduce a novel method to measure absolute blood flow velocity based on high speed spectral domain optical coherence tomography (OCT) and apply it to measure velocities across the heart outflow tract (OFT) of a chicken embryo (stage HH18). First, we use the OCT system to acquire 4D [(x,y,z) + t] images of the OFT in vivo. Second, we reconstruct the 4D microstructural images and obtain the orientation of the OFT at its maximum expansion, from which the centerline of the OFT is calculated based on the OFT boundary segmentation. Assuming flow is parallel to the vessel orientation, the obtained centerline indicates the flow direction. Finally, the absolute flow velocity is evaluated based on the direction given by the centerline and the axial velocity obtained from Doppler OCT. Using this method, we compare flow velocity profiles at various positions along the chicken embryo OFT. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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