Biodegradation of 2-ethylhexyl nitrate (2-EHN) by Mycobacterium austroafricanum IFP 2173
| Autor: | Nicolau, Elodie, Kerhoas, L., Lettere, Martine, Jouanneau, Yves, Marchal, Rémy |
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| Přispěvatelé: | Laboratoire de Chimie et Biologie des Métaux (LCBM - UMR 5249), Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS)-Université Grenoble Alpes [2016-2019] (UGA [2016-2019])-Institut de Recherche Interdisciplinaire de Grenoble (IRIG), Direction de Recherche Fondamentale (CEA) (DRF (CEA)), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Direction de Recherche Fondamentale (CEA) (DRF (CEA)), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA), IFP Energies nouvelles (IFPEN), INRA (INRA), Institut National de la Recherche Agronomique (INRA), Direction Chimie et Physico-chimie appliquées (DCPA), IFP Energies nouvelles (IFPEN)-IFP Energies nouvelles (IFPEN), Collaboration, Centre National de la Recherche Scientifique (CNRS)-Université Grenoble Alpes (UGA)-Institut de Recherche Interdisciplinaire de Grenoble (IRIG), Unité de phytopharmacie et médiateurs chimiques |
| Jazyk: | angličtina |
| Rok vydání: | 2008 |
| Předmět: |
Quantitative Biology - Biomolecules
FOS: Biological sciences branched alkane alkane hydroxylation beta oxidation pathway Biomolecules (q-bio.BM) [SDV.BBM.BC]Life Sciences [q-bio]/Biochemistry Molecular Biology/Biochemistry [q-bio.BM] biodegradation [SDV.BBM.BC]Life Sciences [q-bio]/Biochemistry Molecular Biology/Biomolecules [q-bio.BM] |
| Zdroj: | Applied and Environmental Microbiology Applied and Environmental Microbiology, American Society for Microbiology, 2008, 74, pp.6187-6193 Applied and Environmental Microbiology, 2008, 74, pp.6187-6193. ⟨10.1128/AEM.01142-08⟩ |
| ISSN: | 0099-2240 1098-5336 |
| DOI: | 10.1128/AEM.01142-08⟩ |
| Popis: | International audience; 2-Ethyhexyl nitrate (2-EHN) is a major additive of fuel which is used to comply with the cetane number of diesel. Because of its wide use and possible accidental release, 2-EHN is a potential pollutant of the environment. In this study, Mycobacterium austroafricanum IFP 2173 was selected among several strains as the best 2-EHN degrader. The 2-EHN biodegradation rate was increased in biphasic cultures where the hydrocarbon was dissolved in an inert non-aqueous phase liquid (NAPL), suggesting that the transfer of the hydrophobic substrate to the cells was a growth-limiting factor. Carbon balance calculation as well as organic carbon measurement indicated a release of metabolites in the culture medium. Further analysis by gas chromatography revealed that a single metabolite accumulated during growth. This metabolite had a molecular mass of 114 Da as determined by GC/MS and was provisionally identified as 4-ethyldihydrofuran-2(3H)-one by LC-MS/MS analysis. Identification was confirmed by analysis of the chemically synthesized lactone. Based on these results, a plausible catabolic pathway is proposed whereby 2-EHN is converted to 4-ethyldihydrofuran-2(3H)-one, which cannot be metabolised further by strain IFP 2173. This putative pathway provides an explanation for the low energetic efficiency of 2-EHN degradation and its poor biodegradability. |
| Databáze: | OpenAIRE |
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