Toxocara canis larvae reinfecting BALB/c mice exhibit accelerated speed of migration to the host CNS
| Autor: | David Větvička, Karl Skírnisson, Martin Syrůček, Helena Mareckova, Petra Kolbeková, Libuše Kolářová, Markéta Leissová, Jan Svoboda |
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| Rok vydání: | 2011 |
| Předmět: |
Time Factors
genetic structures Eye Diseases Population Antibodies Helminth Enzyme-Linked Immunosorbent Assay Eye Parasite Load Microbiology BALB/c Toxocara cati chemistry.chemical_compound Mice Antigen parasitic diseases Animals education education.field_of_study Mice Inbred BALB C Central Nervous System Helminthiasis Toxocariasis General Veterinary biology Staining and Labeling fungi Brain Toxocara canis Carboxyfluorescein succinimidyl ester General Medicine biology.organism_classification Infectious Diseases Canis chemistry Insect Science Immunoglobulin G Larva Immunology biology.protein Parasitology Female Antibody |
| Zdroj: | Parasitology research. 109(5) |
| ISSN: | 1432-1955 |
| Popis: | Using a small animal imaging system, migratory activity of Toxocara canis larvae stained by carboxyfluorescein succinimidyl ester (CFSE) was observed post primary infection (PPI) and post reinfection (PR) of BALB/c mice. Each infection was performed with 1,000 larvae per mouse. Primary infections were performed with labeled larvae, while for challenge infections the reinfecting larvae were stained by CFSE. The worm burden in mouse organs was determined during a period from 6 h to 21 days and 4 months PPI and PR. In comparison with primary infections that led to the first larvae appearance in the brain after 60 h, greatly accelerated migration of the parasites administered 3 weeks PPI to the CNS and eyes of challenged mice was noted—in both organs the larvae appeared 6 h PR. In all challenged mice, reinfecting larvae prevailed in the resident parasite population. Preliminary experiments with Toxocara cati larvae also revealed early brain involvement in primarily infected mice. Staining of T. canis larvae by CFSE had no effect on the development of a humoral antibody response against T. canis excretory–secretory antigens. In ELISA, elevated levels of specific IgG and IgG1 were noted on day 14 PPI and the levels of antibodies increased till the end of experiment. Reinfection induced an increase in the levels of both antibodies. In terms of optical density, IgG1 antibodies gave higher values in all sera examined. In ELISA for IgG antibodies, an increase in the avidity index of around 50% was detected 1 month PPI; higher-avidity antibodies were also detected in sera of reinfected animals. |
| Databáze: | OpenAIRE |
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