Erianthus arundinaceus HSP70 (EaHSP70) overexpression increases drought and salinity tolerance in sugarcane (Saccharum spp. hybrid)
| Autor: | Sruthy Maria Augustine, V. Ravichandran, Chinnaswamy Appunu, M. Chakravarthi, J. Ashwin Narayan, N. Subramonian, Divya P. Syamaladevi |
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| Rok vydání: | 2015 |
| Předmět: |
biology
Drought tolerance Water Salt Tolerance Plant Science General Medicine Photosynthetic efficiency Plants Genetically Modified biology.organism_classification Acclimatization Droughts Saccharum Salinity chemistry.chemical_compound Agronomy chemistry Germination Chlorophyll Heat shock protein Genetics HSP70 Heat-Shock Proteins Agronomy and Crop Science |
| Zdroj: | Plant Science. 232:23-34 |
| ISSN: | 0168-9452 |
| DOI: | 10.1016/j.plantsci.2014.12.012 |
| Popis: | Heat shock proteins (HSPs) have a major role in stress tolerance mechanisms in plants. Our studies have shown that the expression of HSP70 is enhanced under water stress in Erianthus arundinaceus. In this paper, we evaluate the effects of overexpression of EaHSP70 driven by Port Ubi 2.3 promoter in sugarcane. The transgenic events exhibit significantly higher gene expression, cell membrane thermostability, relative water content, gas exchange parameters, chlorophyll content and photosynthetic efficiency. The overexpression of EaHSP70 transgenic sugarcane led to the upregulation of stress-related genes. The transformed sugarcane plants had better chlorophyll retention and higher germination ability than control plants under salinity stress. Our results suggest that EaHSP70 plays an important role in sugarcane acclimation to drought and salinity stresses and its potential for genetic engineering of sugarcane for drought and salt tolerance. |
| Databáze: | OpenAIRE |
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