Protecting HaCaT cells from ionizing radiation using persimmon tannin-Aloe gel composite

Autor:	Gao Lin, Jinliang Ning, Na He, Zhide Zhou, Chaoke Qin, Xi Qian, Zhongmin Wang, Guiyin Li, Da-Hong Lin
Rok vydání:	2020
Předmět:	Cell Survival human epidermal keratinocytes cells Pharmaceutical Science Context (language use) RM1-950 x-rays 030226 pharmacology & pharmacy 01 natural sciences Aloe vera Ionizing radiation 03 medical and health sciences 0302 clinical medicine Radiation Ionizing Drug Discovery HaCaT Cells Humans Tannin Asphodelaceae Aloe Pharmacology chemistry.chemical_classification radiation resistance Dose-Response Relationship Drug biology Traditional medicine Plant Extracts Diospyros kaki General Medicine Diospyros biology.organism_classification 0104 chemical sciences 010404 medicinal & biomolecular chemistry HaCaT Complementary and alternative medicine chemistry Cytoprotection Molecular Medicine Therapeutics. Pharmacology Gels Tannins Ebenaceae Research Article
Zdroj:	Pharmaceutical Biology, Vol 58, Iss 1, Pp 510-517 (2020) Pharmaceutical Biology article-version (VoR) Version of Record
ISSN:	1744-5116 1388-0209
DOI:	10.1080/13880209.2020.1767158
Popis:	Context Persimmon tannin (extract of Diospyros kaki L.f [Ebenaceae]) and Aloe gel (extract of Aloe vera (L.) Burm.f. [Asphodelaceae]) are known as anti-radiation agents. However, radiation resistance of the persimmon tannin-Aloe gel composite remains inconclusive. Objective To investigate the capacity of the persimmon tannin-Aloe gel composite to protect against ionising radiation at the cellular level. Materials and methods HaCaT (human epidermal keratinocytes) cells were pre-treated with PT-A-1 (the mass ratio of persimmon tannin and Aloe gel was 2:1) or the single component (persimmon tannin or Aloe gel) at various concentrations (0, 50, 100, 200, 400, 800 μg/mL. Control group: medium with no HaCaT cells), and then radiated with X-rays (radiation dose: 4, 8, 12, 16, and 20 Gy). Cell viability, cell apoptosis, and radiation-induced intracellular reactive oxygen species (ROS) generation were analysed by CCK-8, Hoechst 33258 staining/flow cytometry, and 2′,7′-dichlorfluorescein diacetate (DCFH-DA) assay, respectively, for 12 or 24 h incubation after radiation. Results The optimal radiation dose and post-radiation incubation period were determined to be 8 Gy and 12 h. CCK-8 activity detection showed that the cell activity was 77.85% (p
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_dedup___::a9f581c0a198211953ef8a1faedbdf78 https://doi.org/10.1080/13880209.2020.1767158 Zobrazit plný text záznamu Plný text ve formátu PDF Plný text ve formátu HTML
Nepřihlášeným uživatelům se plný text nezobrazuje	K zobrazení výsledku je třeba se přihlásit.