Antisera reactive directly to estrone sulfate
| Autor: | Michael Su Fah Wong, R.M. Hoskinson, R.I. Cox |
|---|---|
| Rok vydání: | 1979 |
| Předmět: |
Estrone
medicine.medical_treatment Clinical Biochemistry Cross Reactions Biochemistry Steroid Epitopes chemistry.chemical_compound Endocrinology Estrone sulfate medicine Bovine serum albumin Sulfate Molecular Biology Pharmacology Antiserum Chromatography biology Chemistry Immune Sera Organic Chemistry Serum Albumin Bovine Radioimmunoassay biology.protein Haptens Hapten |
| Zdroj: | Steroids. 33:549-562 |
| ISSN: | 0039-128X |
| DOI: | 10.1016/0039-128x(79)90035-7 |
| Popis: | Derivatives of estrone were prepared and linked to bovine serum albumin or its methyl-esterified form to produce immunogens which were effective in raising antisera to estrone sulfate. The most effective was estrone-3-methylphosphonothioate, electrostatically complexed with methylated bovine serum albumin. The ionically combined hapten functioned as an antigenic determinant as do covalently bound haptens when administered to sheep in emulsions with Freund's complete adjuvant. Estrone-3-phosphate covalently or electrostatically linked to bovine serum albumin also produced antisera reactive to estrone sulfate. Estrone sulfate itself, after electrostatically complexing to methylated bovine serum albumin and administration with Freund's complete adjuvant to sheep, was ineffective in producing antisera. The sera which had workable titres to estrone sulfate showed considerable cross-reaction with free estrone but was otherwise highly specific with little or no reaction with other steroid sulfates, glucosiduronates or other free steroids. Radioimmunoassay curves using [6,7- 3 H]-estrone sulfate were highly sensitive and were effective in the range of 5–250 pg estrone sulfate. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|