Difenoconazole induces oxidative DNA damage and mitochondria mediated apoptosis in SH-SY5Y cells
| Autor: | Yang Zhang, Liming Tao, Jiagao Cheng, Bing Wu, Wenping Xu, Te Xie, Hongfei Ni, Zhong Li, Xin Wang, Cheng Zhang |
|---|---|
| Rok vydání: | 2021 |
| Předmět: |
Programmed cell death
Environmental Engineering SH-SY5Y DNA damage Cell Survival Health Toxicology and Mutagenesis Poly ADP ribose polymerase 0208 environmental biotechnology Apoptosis 02 engineering and technology 010501 environmental sciences 01 natural sciences Cell Line Tumor Environmental Chemistry Humans Viability assay Cytotoxicity 0105 earth and related environmental sciences Cell growth Chemistry Public Health Environmental and Occupational Health Dioxolanes General Medicine General Chemistry Triazoles Pollution 020801 environmental engineering Cell biology Mitochondria Oxidative Stress Reactive Oxygen Species |
| Zdroj: | Chemosphere. 283 |
| ISSN: | 1879-1298 |
| Popis: | Difenoconazole is one of the most typical triazole fungicides. Difenoconazole is widely used in the field of agricultural production, and its health and safety problems need to be further studied. The main purpose of this paper is to verify the neurotoxicity of Difenoconazole at the cellular level. In this study, SH-SY5Y cell line of human neuroblastoma was used to evaluate its potentially toxic effects and molecular mechanism in vitro. The research indicated that Difenoconazole could reduce cell viability and inhibit cell proliferation, induce DNA damage and accelerate programmed cell death. Further studies showed that Difenoconazole induced DNA double-strand breaks, intracellular generation of ROS, cleaved PARP, mitochondrial membrane potential collapse, induced Cyt c release, and Bax/Bcl-2 ratio increase in SH-SY5Y cells. In conclusion, the cytotoxicity of Difenoconazole revealed its toxic effect on SH-SY5Y cells, and the IC50 value was 55.41 μM after 24 h exposure. Meanwhile, the genetic toxicity of Difenoconazole has revealed that it can induce DNA damage and apoptosis of SH-SY5Y cells. Through this study, the toxic effects of Difenoconazole on SH-SY5Y cells are further understood, which provides a more scientific basis for its safe use and risk control. |
| Databáze: | OpenAIRE |
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