Efficient production of bioactive structured lipids by fast acidolysis catalyzed by Yarrowia lipolytica lipase, free and immobilized in chitosan-alginate beads, in solvent-free medium
| Autor: | Priscilla Filomena Fonseca Amaral, Emília Akil, Alexandre G. Torres, Tatiana El-Bacha, Adejanildo da S. Pereira |
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| Rok vydání: | 2020 |
| Předmět: |
Alginates
Drug Compounding Lipolysis Yarrowia Chemistry Techniques Synthetic 02 engineering and technology Biochemistry Catalysis Chitosan 03 medical and health sciences chemistry.chemical_compound Structural Biology Triolein Lipase Olive Oil Molecular Biology 030304 developmental biology chemistry.chemical_classification 0303 health sciences Chromatography Esterification biology Fatty Acids Fatty acid General Medicine Hydrogen-Ion Concentration Enzymes Immobilized 021001 nanoscience & nanotechnology biology.organism_classification Lipids Lauric acid Microspheres Enzyme Activation chemistry Biocatalysis Dietary Supplements biology.protein lipids (amino acids peptides and proteins) 0210 nano-technology |
| Zdroj: | International Journal of Biological Macromolecules. 163:910-918 |
| ISSN: | 0141-8130 |
| DOI: | 10.1016/j.ijbiomac.2020.06.282 |
| Popis: | Structured lipids (SL) represent a new generation of lipids, considered bioactive compounds. Medium-chain, oleic (18:1n-9), and medium-chain fatty acid (MCFA) structured lipids (MOM-SL) were produced by acidolysis reaction in solvent-free medium with capric (10:0) and lauric (12:0) free fatty acids (FFAs) and triolein or olive oil, using Yarrowia lipolytica lipase as biocatalyst. MCFAs were rapidly incorporated into sn-1,3 SL in acidolysis reactions with triolein and olive oil, up until 30% of incorporation efficiency of capric and lauric acids in SLs. The kinetics of MCFA incorporation in MOM-SL was influenced by the FFA:TAG molar ratio, and for reactions between triolein and lauric acid, increasing FFA:TAG from 2:1 to 4:1 enhanced MCFA incorporation in SL. Y. lipolytica lipase showed a strictly 1,3-regioselective profile in acidolysis reaction, confirmed by nuclear magnetic resonance spectroscopy. Immobilization of this lipase by microencapsulation in chitosan-alginate beads resulted in similar incorporation efficiency for lauric acid with olive oil TAG and this reaction could be performed for 5 cycles without catalytic activity loss. This lipase showed promising properties as a potential biocatalyst that may be effectively used in production of bioactive structured lipids, which might be applied for prevention of metabolic and inflammatory disorders related to obesity. |
| Databáze: | OpenAIRE |
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