Targeted diversity generation by intraterrestrial archaea and archaeal viruses
| Autor: | Jeff F. Miller, Sarah C. Bagby, Alexander Sczyrba, David L. Valentine, Elizabeth Czornyj, Blair G. Paul, Sumit Handa, Partho Ghosh, Diego Arambula |
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| Rok vydání: | 2015 |
| Předmět: |
Archaeal Viruses
Mutation rate Retroelements Archaeal Proteins Molecular Sequence Data General Physics and Astronomy Biology 2.2 Factors relating to physical environment Genome Article General Biochemistry Genetics and Molecular Biology Vaccine Related 03 medical and health sciences Mutation Rate Biodefense Nanoarchaeota Genetics 2.2 Factors relating to the physical environment Aetiology 030304 developmental biology 0303 health sciences Multidisciplinary Base Sequence 030306 microbiology Prevention Genetic Variation General Chemistry biology.organism_classification Archaea Reverse transcriptase Emerging Infectious Diseases Infectious Diseases Novel virus Metagenome Infection Bacteria |
| Zdroj: | Nature communications, vol 6, iss 1 Paul, BG; Bagby, SC; Czornyj, E; Arambula, D; Handa, S; Sczyrba, A; et al.(2015). Targeted diversity generation by intraterrestrial archaea and archaeal viruses. Nature Communications, 6. doi: 10.1038/ncomms7585. UC San Diego: Retrieved from: http://www.escholarship.org/uc/item/90z6x2r3 Nature Communications |
| Popis: | In the evolutionary arms race between microbes, their parasites, and their neighbours, the capacity for rapid protein diversification is a potent weapon. Diversity-generating retroelements (DGRs) use mutagenic reverse transcription and retrohoming to generate myriad variants of a target gene. Originally discovered in pathogens, these retroelements have been identified in bacteria and their viruses, but never in archaea. Here we report the discovery of intact DGRs in two distinct intraterrestrial archaeal systems: a novel virus that appears to infect archaea in the marine subsurface, and, separately, two uncultivated nanoarchaea from the terrestrial subsurface. The viral DGR system targets putative tail fibre ligand-binding domains, potentially generating >1018 protein variants. The two single-cell nanoarchaeal genomes each possess ≥4 distinct DGRs. Against an expected background of low genome-wide mutation rates, these results demonstrate a previously unsuspected potential for rapid, targeted sequence diversification in intraterrestrial archaea and their viruses. Diversity-generating retroelements (DGRs) are genetic elements that introduce sequence variation within target genes in bacteria and their viruses. Here, Paul et al. report the discovery of DGRs in an archaeal virus and in two archaea from marine and terrestrial subsurface environments, respectively. |
| Databáze: | OpenAIRE |
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