Polyacetylene diols with antiproliferative and driving Th1 polarization effects from the marine sponge Callyspongia sp
| Autor: | Noboru Shoji, Shigenobu Arihara, Toshihiro Hashimoto, Miwa Matsui, Eri Arimoto, Akemi Nakata, Masao Takei, Nanae Matsuoka, Rieko Mine, Akemi Umeyama |
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| Rok vydání: | 2009 |
| Předmět: |
Polymers
Stereochemistry Diol Antineoplastic Agents Apoptosis HL-60 Cells Fractionation Th1 polarization Inhibitory Concentration 50 Polyacetylene chemistry.chemical_compound Leukemia Promyelocytic Acute Animals Humans Cell Proliferation biology Acetylene Cell Polarity Polyynes Cell Differentiation Dendritic Cells Th1 Cells biology.organism_classification Callyspongia Sponge Biochemistry chemistry Molecular Medicine Drug Screening Assays Antitumor Callyspongiidae |
| Zdroj: | Journal of Natural Medicines. 64:93-97 |
| ISSN: | 1861-0293 1340-3443 |
| DOI: | 10.1007/s11418-009-0363-3 |
| Popis: | A screening of 30 crude extracts of marine sponges against human promyelocytic leukemia cells (HL-60) yielded an EtOAc extract of the sponge Callyspongia sp. (Callyspongiidae) with significant activity. Further bioassay-guided fractionation of the EtOAc extract led to the isolation of three polyacetylene metabolites: a new polyacetylene diol, callyspongidiol (1), along with two known compounds, siphonodiol (2) and 14,15-dihydrosiphonodiol (3). Their structures were determined by a combination of spectroscopic analyses. Compounds 1-3 exhibited antiproliferative activity against HL-60 with IC(50) values of 6.5, 2.8, and 6.5 microg/ml, respectively. These metabolites induce apoptosis in HL-60 cells. Dendritic cells (DC) differentiated with 1-3 enhance the differentiation of naïve T cells towards the Th1 type. |
| Databáze: | OpenAIRE |
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