Human Osteoblast-Derived Extracellular Matrix with High Homology to Bone Proteome Is Osteopromotive
| Autor: | Yik Y. Kan, Marta Baroncelli, Johannes P.T.M. van Leeuwen, Enrique Rull Trinidad, Marijke Koedam, Jeroen Demmers, Bram C. J. van der Eerden, Jeroen van de Peppel, Ingmar A.J. van Hengel, Siddharth Chatterji, Rodrigo D. A. M. Alves, Lidy E. Fratila-Apachitei |
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| Přispěvatelé: | Internal Medicine, Biochemistry |
| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
Ceramics Proteome Biomedical Engineering Bioengineering Mice SCID 02 engineering and technology Mesenchymal Stem Cell Transplantation Biochemistry Bone and Bones Cell Line Biomaterials Extracellular matrix 03 medical and health sciences Ectopic calcification Calcification Physiologic Mice Inbred NOD In vivo medicine Animals Humans Bone regeneration Osteoblasts Chemistry Mesenchymal stem cell Cell Differentiation Mesenchymal Stem Cells Osteoblast 021001 nanoscience & nanotechnology medicine.disease In vitro Extracellular Matrix Cell biology 030104 developmental biology medicine.anatomical_structure Heterografts Glass 0210 nano-technology |
| Zdroj: | Tissue Engineering Part A, 24(17-18), 1377-1389. Mary Ann Liebert Inc. |
| ISSN: | 1937-335X 1937-3341 |
| Popis: | Efficient osteogenic differentiation of mesenchymal stromal cells (MSCs) is crucial to accelerate bone formation. In this context, the use of extracellular matrix (ECM) as natural 3D framework mimicking in vivo tissue architecture is of interest. The aim of this study was to generate a devitalized human osteogenic MSC-derived ECM and to investigate its impact on MSC osteogenic differentiation to improve MSC properties in bone regeneration. The devitalized ECM significantly enhanced MSC adhesion and proliferation. Osteogenic differentiation and mineralization of MSCs on the ECM were quicker than in standard conditions. The presence of ECM promoted in vivo bone formation by MSCs in a mouse model of ectopic calcification. We analyzed the ECM composition by mass spectrometry, detecting 846 proteins. Of these, 473 proteins were shared with the human bone proteome we previously described, demonstrating high homology to an in vivo microenvironment. Bioinformatic analysis of the 846 proteins showed involvement in adhesion and osteogenic differentiation, confirming the ECM composition as key modulator of MSC behavior. In addition to known ECM components, proteomic analysis revealed novel ECM functions, which could improve culture conditions. In summary, this study provides a simplified method to obtain an in vitro MSC-derived ECM that enhances osteogenic differentiation and could be applied as natural biomaterial to accelerate bone regeneration. |
| Databáze: | OpenAIRE |
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