Method validation and simultaneous quantification of eight organochlorines/organophosphates in apple by gas chromatography
| Autor: | Ishrat Jan, Alamgir A. Dar, Irshad H. Dar, Ashraf Alam Wani, Khurshid A. Sofi, J. A. Sofi |
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| Rok vydání: | 2019 |
| Předmět: |
Chromatography
Gas 030309 nutrition & dietetics Calibration curve Ethion Cypermethrin 03 medical and health sciences chemistry.chemical_compound 0404 agricultural biotechnology Limit of Detection Pyrethrins Hydrocarbons Chlorinated Mathematics 0303 health sciences Residue (complex analysis) Nutrition and Dietetics Chromatography Pesticide residue Organothiophosphates Pesticide Residues 04 agricultural and veterinary sciences Pesticide 040401 food science Organophosphates chemistry Fruit Malus Chlorpyrifos Gas chromatography Agronomy and Crop Science Food Science Biotechnology |
| Zdroj: | Journal of the Science of Food and Agriculture. 99:3687-3692 |
| ISSN: | 1097-0010 0022-5142 |
| Popis: | BACKGROUND: The increasing and extensive use of pesticides worldwide has resulted in a significant loss of non‐target populations particularly humans by direct or indirect exposures. Also, various methods have been used for the estimation of pesticide residues in fruits and vegetables from recent past which are either tedious, time consuming or expensive. Therefore, the present study was performed to determine the pesticide residues from apple by simple and novel validated gas chromatography. RESULTS: A novel, accurate, ecofriendly and cost‐effective gas chromatography method was developed for simultaneous quantification of eight pesticides, namely chlorpyrifos‐methyl (1), chlorpyrifos (2), quinolphos (3), profenofos (4), myclobutnil (5), ethion (6), fenpropathrin (7) and cypermethrin (8). The developed method was validated as per the SANTE guidelines. All calibration curves showed a good linear relationship (r > 0.99) within the test range. Precision was evaluated by intra‐ and inter‐day tests with relative standard deviations (RSDs) < 2.0%, recovery in between 70% and 120% with RSDs < 2.00%. CONCLUSION: The results demonstrate that the concentration of pesticides 1 to 8 were found below the detectable limit. Method validation parameters like linearity, precision, accuracy, specificity, robustness, detection and quantification limits were found within the acceptable range. The proposed method makes it possible to determine simultaneously pesticides 1–8 in one run which can be extended for residue‐based standardization of pesticides from apple and other fruits and vegetables. © 2019 Society of Chemical Industry |
| Databáze: | OpenAIRE |
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