Determination of vitamin E in aquatic organisms by high-performance liquid chromatography with fluorescence detection
| Autor: | Patrick Sorgeloos, André P. De Leenheer, Hans Nelis, Patrick Lavens, Ji-Zeng Huo |
|---|---|
| Rok vydání: | 1996 |
| Předmět: |
Antioxidant
medicine.medical_treatment Biophysics Rotifera Biochemistry High-performance liquid chromatography Fluorescence chemistry.chemical_compound medicine Butylated hydroxytoluene Animals Vitamin E Sea bass Molecular Biology Chromatography High Pressure Liquid Chromatography biology Methanol Extraction (chemistry) Cell Biology Butylated Hydroxytoluene biology.organism_classification Turbot chemistry Larva Flatfishes Artemia alpha-Tocopherol |
| Zdroj: | Analytical biochemistry. 242(1) |
| ISSN: | 0003-2697 |
| Popis: | A liquid chromatographic (HPLC) method has been developed for the quantitative determination of different forms of vitamin E (alpha-, gamma-, and delta-tocopherol) in aquatic organisms. The assay consists of a simple extraction with methanol containing butylhydroxytoluene (BHT) as an antioxidant, followed by reversed phase chromatography with fluorescence detection. The efficiency of the extraction method was equivalent or superior to that of more complex approaches for the isolation of tocopherols. Linearity has been achieved over the range of 0.02 to 3 µg/ml for alpha-tocopherol and within-run and between-run coefficients of variation at three levels were 0.7-2.9 and 1.2-3.7%, respectively. The recovery at three concentrations ranged from 73.8 to 96.6% and the minimal quantity that could be detected was 0.6 ng. Comparable figures were obtained for gamma- and delta-tocopherol. This method has been routinely applied to determine vitamin E in Artemia , rotifers, turbot and sea bass larvae, and shrimp postlarvae. |
| Databáze: | OpenAIRE |
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